J Biosci Bioeng. 2011 Dec;112(6):595-601. Improving protein secretion of a transglutaminase-secreting Corynebacterium glutamicum recombinant strain on the basis of 13C metabolic flux analysis. Umakoshi M, Hirasawa T, Furusawa C, et al.
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| ATP production rate Maximum | 346.05 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 317.05 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 103.4 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 91.8 | R02736+R01528 |
| ATP production rate Maximum | 300.65 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 285.65 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 97.8 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 91.8 | R02736+R01528 |
| ATP production rate Maximum | 594.7 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 508.2 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 114.6 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 80 | R02736+R01528 |
| ATP production rate Maximum | 386.3 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 278.3 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 156 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 112.8 | R02736+R01528 |
| ATP production rate Maximum | 434.05 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 295.8 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 153.1 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 97.8 | R02736+R01528 |
| ATP production rate Maximum | 329.6 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 224.6 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 84.6 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 42.6 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum YDK010/pPSPTG11 |
| Genotype | secreting TGase |
| Culture medium | For precultivation,CM-2G medium consisting of 1% polypeptone, 1% yeast extract, 0.5% NaCl, and 0.5% glucose (pH7.2,adjusted by the addition of KOH) was used. For the main batch cultivation,modified MMTG medium consisting of 60 g/L glucose,3 g/L (NH4)2SO4,3 g/L MgSO4-7H2O,1.5 g/L KH2PO4,30 mg/L FeSO4-7H2O,30 mg/L MnSO4-5H2O,0.2 g/L CaCl2,and 0.45 mg/L biotin was used. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Metabolic flux distributions of the TGase-secreting strain 9h after inoculation. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum YDK010/pPSPTG11 |
| Genotype | secreting TGase |
| Culture medium | For precultivation,CM-2G medium consisting of 1% polypeptone, 1% yeast extract, 0.5% NaCl, and 0.5% glucose (pH7.2,adjusted by the addition of KOH) was used. For the main batch cultivation,modified MMTG medium consisting of 60 g/L glucose,3 g/L (NH4)2SO4,3 g/L MgSO4-7H2O,1.5 g/L KH2PO4,30 mg/L FeSO4-7H2O,30 mg/L MnSO4-5H2O,0.2 g/L CaCl2,and 0.45 mg/L biotin was used. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Metabolic flux distributions of the TGase-secreting strain 12h after inoculation. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum YDK010/pPSPTG11 |
| Genotype | secreting TGase |
| Culture medium | For precultivation,CM-2G medium consisting of 1% polypeptone, 1% yeast extract, 0.5% NaCl, and 0.5% glucose (pH7.2,adjusted by the addition of KOH) was used. For the main batch cultivation,modified MMTG medium consisting of 60 g/L glucose,3 g/L (NH4)2SO4,3 g/L MgSO4-7H2O,1.5 g/L KH2PO4,30 mg/L FeSO4-7H2O,30 mg/L MnSO4-5H2O,0.2 g/L CaCl2,and 0.45 mg/L biotin was used. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Metabolic flux distributions of the TGase-secreting strain 15h after inoculation. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum YDK010/pPK4 |
| Genotype | carrying empty vector |
| Culture medium | For precultivation,CM-2G medium consisting of 1% polypeptone, 1% yeast extract, 0.5% NaCl, and 0.5% glucose (pH7.2,adjusted by the addition of KOH) was used. For the main batch cultivation,modified MMTG medium consisting of 60 g/L glucose,3 g/L (NH4)2SO4,3 g/L MgSO4-7H2O,1.5 g/L KH2PO4,30 mg/L FeSO4-7H2O,30 mg/L MnSO4-5H2O,0.2 g/L CaCl2,and 0.45 mg/L biotin was used. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Metabolic flux distributions of empty vector-carrying strain 9h after inoculation. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum YDK010/pPK4 |
| Genotype | carrying empty vector |
| Culture medium | For precultivation,CM-2G medium consisting of 1% polypeptone, 1% yeast extract, 0.5% NaCl, and 0.5% glucose (pH7.2,adjusted by the addition of KOH) was used. For the main batch cultivation,modified MMTG medium consisting of 60 g/L glucose,3 g/L (NH4)2SO4,3 g/L MgSO4-7H2O,1.5 g/L KH2PO4,30 mg/L FeSO4-7H2O,30 mg/L MnSO4-5H2O,0.2 g/L CaCl2,and 0.45 mg/L biotin was used. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Metabolic flux distributions of empty vector-carrying strain 12h after inoculation. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum YDK010/pPK4 |
| Genotype | carrying empty vector |
| Culture medium | For precultivation,CM-2G medium consisting of 1% polypeptone, 1% yeast extract, 0.5% NaCl, and 0.5% glucose (pH7.2,adjusted by the addition of KOH) was used. For the main batch cultivation,modified MMTG medium consisting of 60 g/L glucose,3 g/L (NH4)2SO4,3 g/L MgSO4-7H2O,1.5 g/L KH2PO4,30 mg/L FeSO4-7H2O,30 mg/L MnSO4-5H2O,0.2 g/L CaCl2,and 0.45 mg/L biotin was used. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Metabolic flux distributions of empty vector-carrying strain 15h after inoculation. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.4 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.1 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.7 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.9 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.3 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.7 |