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FEMS Microbiol Lett. 2004 Jun;235(1):17-23. Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements. Peng L, Arauzo-Bravo MJ, Shimizu K.



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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 571.1 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 53.6 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 951.3 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 30.2 R02736+R01528
Growth Condition
Strains Escherichia coli BW25113
Genotype laclq rrnBT14 delta lacZWJ16 hsdR514 delta araBADAH33 rhaBADLD78
Culture medium M9 minimal medium with the exception that the glucoseconcentrations: 5 g/L for batch cultivation and 4g/L for chemostat culture.
Carbon source Glucose
Growth rate 0.41/h
Case-specific description Metabolic flux distribution in the chemostat culture of the wild type E.coli.
Growth Condition
Strains Escherichia coli JWK3928
Genotype ppc mutant
Culture medium M9 minimal medium with the exception that the glucoseconcentrations: 5 g/L for batch cultivation and 4 g/L for chemostat culture.
Carbon source Glucose
Growth rate 0.34/h
Case-specific description Metabolic flux distribution in the chemostat culture of the ppc mutant E.coli.
Specific Rate (mmol g-1 h-1)
glucose 5.61
CO2 3.38
Specific Rate (mmol g-1 h-1)
glucose 3.16
CO2 2.47
Source