FEMS Microbiol Lett. 2004 Jun;235(1):17-23. Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements. Peng L, Arauzo-Bravo MJ, Shimizu K.
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ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | 571.1 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 53.6 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | 951.3 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 30.2 | R02736+R01528 |
Growth Condition | |
Strains | Escherichia coli BW25113 |
Genotype | laclq rrnBT14 delta lacZWJ16 hsdR514 delta araBADAH33 rhaBADLD78 |
Culture medium | M9 minimal medium with the exception that the glucoseconcentrations: 5 g/L for batch cultivation and 4g/L for chemostat culture. |
Carbon source | Glucose |
Growth rate | 0.41/h |
Case-specific description | Metabolic flux distribution in the chemostat culture of the wild type E.coli. |
Growth Condition | |
Strains | Escherichia coli JWK3928 |
Genotype | ppc mutant |
Culture medium | M9 minimal medium with the exception that the glucoseconcentrations: 5 g/L for batch cultivation and 4 g/L for chemostat culture. |
Carbon source | Glucose |
Growth rate | 0.34/h |
Case-specific description | Metabolic flux distribution in the chemostat culture of the ppc mutant E.coli. |
Specific Rate | (mmol g-1 h-1) |
glucose | 5.61 |
CO2 | 3.38 |
Specific Rate | (mmol g-1 h-1) |
glucose | 3.16 |
CO2 | 2.47 |