Appl Environ Microbiol. 2004 Apr;70(4):2307-17. Molecular basis for anaerobic growth of Saccharomyces cerevisiae on xylose, investigated by global gene expression and metabolic flux analysis. Sonderegger M, Jeppsson M, Hahn-Hagerdal B, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 6.96 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 33.5 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 30.18 | R02736+R01528 |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae TMB3001 |
| Genotype | (CEN.PK 113-7A [MATa his3-Delta1 MAL2-8c SUC2] his3::YIpXR/XDH/XK) |
| Culture medium | Cultures were stored in aliquots supplemented with 15% glycerol at -80 Celsius and were revived by growth in YPD medium (10 g of yeast extract per liter, 20 g of peptone per liter, 20 g of glucose per liter). All cultures used for physiological and DNA microarray experiments were grown in minimal medium. For anaerobic cultivation,the medium was supplemented with ethanol-dissolved ergosterol (Fluka) and Tween 80 (Sigma) at final concentrations of 0.01 and 0.42 g/liter, respectively. |
| Carbon source | Glucose |
| Growth rate | 0.05/h |
| Case-specific description | Molar carbon fluxes during anaerobic exponential batch growth of TMB3001 on 10 g of glucose per liter. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae C1 |
| Genotype | (CEN.PK 113-7A [MATa his3-Delta1 MAL2-8c SUC2] his3::YIpXR/XDH/XK) |
| Culture medium | Cultures were stored in aliquots supplemented with 15% glycerol at -80 Celsius and were revived by growth in YPD medium (10 g of yeast extract per liter, 20 g of peptone per liter, 20 g of glucose per liter). All cultures used for physiological and DNA microarray experiments were grown in minimal medium. For anaerobic cultivation,the medium was supplemented with ethanol-dissolved ergosterol (Fluka) and Tween 80 (Sigma) at final concentrations of 0.01 and 0.42 g/liter, respectively. |
| Carbon source | Xylose |
| Growth rate | 0.05/h |
| Case-specific description | Molar carbon fluxes during anaerobic exponential batch growth of C1 on 10 g of xylose per liter. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae C1 |
| Genotype | (CEN.PK 113-7A [MATa his3-Delta1 MAL2-8c SUC2] his3::YIpXR/XDH/XK) |
| Culture medium | Cultures were stored in aliquots supplemented with 15% glycerol at -80 Celsius and were revived by growth in YPD medium (10 g of yeast extract per liter, 20 g of peptone per liter, 20 g of glucose per liter). All cultures used for physiological and DNA microarray experiments were grown in minimal medium. For anaerobic cultivation,the medium was supplemented with ethanol-dissolved ergosterol (Fluka) and Tween 80 (Sigma) at final concentrations of 0.01 and 0.42 g/liter, respectively. |
| Carbon source | Xylose |
| Growth rate | 0.05/h |
| Case-specific description | Molar carbon fluxes during 10 g of xylose per liter plus 0.5 g of acetoin per liter. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.41 |
| xylose | 0.497 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.267 |
| xylose | 0.323 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 3.47 |
| xylose | 4.246 |