J Biotechnol. 2006 Mar;122(2):254-66. Effect of lpdA gene knockout on the metabolism in Escherichia coli based on enzyme activities, intracellular metabolite concentrations and metabolic flux analysis by 13C-labeling experiments. Li M, Ho PY, Yao S,et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli BW25113 |
| Genotype | lacIq rrnBT14 delta lacZWJ16 hsdR514 delta araBADAH33 delta rha-BADLD78 |
| Culture medium | Batch cultivation was conducted first using LB medium containing 8g/L of glucose. For continuous cultivation, the minimal synthetic medium was used. It contained 4 g of glucose per liter(filter sterilized), 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, and 30 mM (NH4)2SO4.The following components were filter sterilized and then added (per liter of final medium): 1 ml of 1 M MgSO4, 1 ml of 0.1 mM CaCl2, 1 ml of 1 mg of Vitamin B1 per liter, and 10 ml of trace element solution containing (per liter) 0.55 g of CaCl2, 1 g of FeCl3, 0.1 g of MnCl2-4H2O, 0.17 g of ZnCl2, 0.043 g of CuCl2-2H2O, 0.06 g of CoCl2-6H2O and 0.06 g Na2MoO4-2H2O. |
| Carbon source | Glucose |
| Growth rate | 0.2/h |
| Case-specific description | Metabolic flux distributions of wild type at dilution rate of 0.2/h. |
| Growth Condition | |
| Strains | Escherichia coli JWK0112 |
| Genotype | lpdA knock-out mutant |
| Culture medium | Batch cultivation was conducted first using LB medium containing 8g/L of glucose. For continuous cultivation, the minimal synthetic medium was used. It contained 4 g of glucose per liter(filter sterilized), 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, and 30 mM (NH4)2SO4.The following components were filter sterilized and then added (per liter of final medium): 1 ml of 1 M MgSO4, 1 ml of 0.1 mM CaCl2, 1 ml of 1 mg of Vitamin B1 per liter, and 10 ml of trace element solution containing (per liter) 0.55 g of CaCl2, 1 g of FeCl3, 0.1 g of MnCl2-4H2O, 0.17 g of ZnCl2, 0.043 g of CuCl2-2H2O, 0.06 g of CoCl2-6H2O and 0.06 g Na2MoO4-2H2O. |
| Carbon source | Glucose |
| Growth rate | 0.22/h |
| Case-specific description | Metabolic flux distributions of lpdA mutant at dilution rate of 0.22/h. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 3.04 |
| CO2 | 8.17 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 2.48 |
| CO2 | 4.08 |