Genome Res. 2005 Oct;15(10):1421-30. Metabolic functions of duplicate genes in Saccharomyces cerevisiae. Kuepfer L, Sauer U, Blank LM.
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| ATP production rate Maximum | -923.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | -938.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 28 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 22 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 32 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 22 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 62 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 30 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 14 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae BY4741 |
| Genotype | (MATa his3 delta1 leu2 delta0 met15 delta0 ura3 delta0) |
| Culture medium | The minimal medium was, per liter (Verduyn et al. 1992), 5 g of (NH4)2SO4, 3 g of KH2PO4, 0.5 g of MgSO4-7H2O, 4.5 mg of ZnSO4-7H2O, 0.3 mg of CoCl2-6H2O, 1.0 mg of MnCl2-4H2O,0.3 mg of CuSO4- 5H2O, 4.5 mg of CaCl2-2H2O, 3.0 mg of FeSO4-7H2O, 0.4 mg of NaMoO4-2H2O, 1.0 mg of H3BO3, 0.1 mg of KI, 15 mg of EDTA, 0.05 mg of biotin, 1.0 mg of Ca-pantothenate, 1.0 mg of nicotinic acid, 25 mg of inositol, 1.0 mg of pyridoxine, 0.2 mg of p-amino-benzoic acid, and 1.0 mg of thiamine. The carbon sources (ethanol, galactose, glucose, and glycerol) were added to a final concentration of 20 g/L. Strainauxotrophies were complemented with 20 mg/L histidine, uracil, methionine, and 60 mg/L leucine. About 50 strains of the yeast collection are lysine auxotroph and were independently tested for growth on plates supplemented with 20 mg/L lysine. The YPD medium consisted, per liter, of 10 g of yeast extract, 20 g ofpeptone, and 20 g of glucose. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Relative distribution of absolute carbon fluxes through S.cerevisiae central carbon metabolism. Flux distributions were obtained with 13C-constrained flux analysis based on the data of Blank and Sauer (2004). |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae BY4741 |
| Genotype | (MATa his3 delta1 leu2 delta0 met15 delta0 ura3 delta0) |
| Culture medium | The minimal medium was, per liter (Verduyn et al. 1992), 5 g of (NH4)2SO4, 3 g of KH2PO4, 0.5 g of MgSO4-7H2O, 4.5 mg of ZnSO4-7H2O, 0.3 mg of CoCl2-6H2O, 1.0 mg of MnCl2-4H2O,0.3 mg of CuSO4- 5H2O, 4.5 mg of CaCl2-2H2O, 3.0 mg of FeSO4-7H2O, 0.4 mg of NaMoO4-2H2O, 1.0 mg of H3BO3, 0.1 mg of KI, 15 mg of EDTA, 0.05 mg of biotin, 1.0 mg of Ca-pantothenate, 1.0 mg of nicotinic acid, 25 mg of inositol, 1.0 mg of pyridoxine, 0.2 mg of p-amino-benzoic acid, and 1.0 mg of thiamine. The carbon sources (ethanol, galactose, glucose, and glycerol) were added to a final concentration of 20 g/L. Strainauxotrophies were complemented with 20 mg/L histidine, uracil, methionine, and 60 mg/L leucine. About 50 strains of the yeast collection are lysine auxotroph and were independently tested for growth on plates supplemented with 20 mg/L lysine. The YPD medium consisted, per liter, of 10 g of yeast extract, 20 g ofpeptone, and 20 g of glucose. |
| Carbon source | Galactose |
| Growth rate | |
| Case-specific description | Relative distribution of absolute carbon fluxes through S.cerevisiae central carbon metabolism. Flux distributions were obtained with stoichiometric flux balancing with galactose. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae BY4741 |
| Genotype | (MATa his3 delta1 leu2 delta0 met15 delta0 ura3 delta0) |
| Culture medium | The minimal medium was, per liter (Verduyn et al. 1992), 5 g of (NH4)2SO4, 3 g of KH2PO4, 0.5 g of MgSO4-7H2O, 4.5 mg of ZnSO4-7H2O, 0.3 mg of CoCl2-6H2O, 1.0 mg of MnCl2-4H2O,0.3 mg of CuSO4- 5H2O, 4.5 mg of CaCl2-2H2O, 3.0 mg of FeSO4-7H2O, 0.4 mg of NaMoO4-2H2O, 1.0 mg of H3BO3, 0.1 mg of KI, 15 mg of EDTA, 0.05 mg of biotin, 1.0 mg of Ca-pantothenate, 1.0 mg of nicotinic acid, 25 mg of inositol, 1.0 mg of pyridoxine, 0.2 mg of p-amino-benzoic acid, and 1.0 mg of thiamine. The carbon sources (ethanol, galactose, glucose, and glycerol) were added to a final concentration of 20 g/L. Strainauxotrophies were complemented with 20 mg/L histidine, uracil, methionine, and 60 mg/L leucine. About 50 strains of the yeast collection are lysine auxotroph and were independently tested for growth on plates supplemented with 20 mg/L lysine. The YPD medium consisted, per liter, of 10 g of yeast extract, 20 g ofpeptone, and 20 g of glucose. |
| Carbon source | Glycerol |
| Growth rate | |
| Case-specific description | Relative distribution of absolute carbon fluxes through S.cerevisiae central carbon metabolism. Flux distributions were obtained with stoichiometric flux balancing with glycerol. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae BY4741 |
| Genotype | (MATa his3 delta1 leu2 delta0 met15 delta0 ura3 delta0) |
| Culture medium | The minimal medium was, per liter (Verduyn et al. 1992), 5 g of (NH4)2SO4, 3 g of KH2PO4, 0.5 g of MgSO4-7H2O, 4.5 mg of ZnSO4-7H2O, 0.3 mg of CoCl2-6H2O, 1.0 mg of MnCl2-4H2O,0.3 mg of CuSO4- 5H2O, 4.5 mg of CaCl2-2H2O, 3.0 mg of FeSO4-7H2O, 0.4 mg of NaMoO4-2H2O, 1.0 mg of H3BO3, 0.1 mg of KI, 15 mg of EDTA, 0.05 mg of biotin, 1.0 mg of Ca-pantothenate, 1.0 mg of nicotinic acid, 25 mg of inositol, 1.0 mg of pyridoxine, 0.2 mg of p-amino-benzoic acid, and 1.0 mg of thiamine. The carbon sources (ethanol, galactose, glucose, and glycerol) were added to a final concentration of 20 g/L. Strainauxotrophies were complemented with 20 mg/L histidine, uracil, methionine, and 60 mg/L leucine. About 50 strains of the yeast collection are lysine auxotroph and were independently tested for growth on plates supplemented with 20 mg/L lysine. The YPD medium consisted, per liter, of 10 g of yeast extract, 20 g ofpeptone, and 20 g of glucose. |
| Carbon source | Ethanol |
| Growth rate | |
| Case-specific description | Relative distribution of absolute carbon fluxes through S.cerevisiae central carbon metabolism. Flux distributions were obtained with stoichiometric flux balancing with ethanol. |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |