Nat Biotechnol. 1997 May;15(5):448-52. Metabolic fluxes in riboflavin-producing Bacillus subtilis. Sauer U, Hatzimanikatis V, Bailey JE, et al.
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| ATP production rate Maximum | 1046 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 793.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 245 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 144 | R02736+R01528 |
| ATP production rate Maximum | 652.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 500 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 189 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 128 | R02736+R01528 |
| ATP production rate Maximum | 615.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 478 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 185 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 130 | R02736+R01528 |
| Growth Condition | |
| Strains | B.subtilis PRF93 |
| Genotype | The recombinant,riboflavin-producing strain is characterized by a single chromosomal copy of the modified B.subtilis rib operon and a deregulating mutation in the ribC gene. |
| Culture medium | The minimal medium was supplemented either with 3.6g/L of glucose containing 13C at natural abundance,or with 3.1g/L of glucose at natural abundance and 0.5g/L uniformly labeled [13C6]-glucose. |
| Carbon source | Glucose |
| Growth rate | 0.11/h |
| Case-specific description | In vivo flux distribution in central carbon metabolism of the riboflavin-producing B.subtilis strain was normallzed to the dilution rate of 0.11/h in the glucose-limited chemostat. |
| Growth Condition | |
| Strains | B.subtilis PRF93 |
| Genotype | The recombinant,riboflavin-producing strain is characterized by a single chromosomal copy of the modified B.subtilis rib operon and a deregulating mutation in the ribC gene. |
| Culture medium | The minimal medium was supplemented either with 3.6g/L of glucose containing 13C at natural abundance,or with 3.1g/L of glucose at natural abundance and 0.5g/L uniformly labeled [13C6]-glucose. |
| Carbon source | Glucose |
| Growth rate | 0.41/h |
| Case-specific description | In vivo flux distribution in central carbon metabolism of the riboflavin-producing B.subtilis strain was normallzed to the dilution rate of 0.41/h in the glucose-limited chemostat. |
| Growth Condition | |
| Strains | B.subtilis PRF93 |
| Genotype | The recombinant,riboflavin-producing strain is characterized by a single chromosomal copy of the modified B.subtilis rib operon and a deregulating mutation in the ribC gene. |
| Culture medium | The minimal medium was supplemented either with 3.6g/L of glucose containing 13C at natural abundance,or with 3.1g/L of glucose at natural abundance and 0.5g/L uniformly labeled [13C6]-glucose. |
| Carbon source | Glucose |
| Growth rate | 0.62/h |
| Case-specific description | In vivo flux distribution in central carbon metabolism of the riboflavin-producing B.subtilis strain was normallzed to the dilution rate of 0.62/h in the glucose-limited chemostat. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.12 |
| O2 | 6.6 |
| CO2 | 6.7 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 5.5 |
| O2 | 13.7 |
| CO2 | 11.4 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 8.27 |
| O2 | 19.8 |
| CO2 | 16.4 |