J Bacteriol. 2004 Mar;186(6):1769-84. In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome. Kromer JO, Sorgenfrei O, Klopprogge K, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 577.88 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 144.18 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 331.81 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 127.28 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 394 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 120 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 492.11 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 105.26 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 13287 |
| Genotype | This homoserine auxotrophic strain was derived from wild-type C.glutamicum ATCC 13032 by UV mutagenesis and selection.It overproduces lysine in the absence of threonine due to the release of aspartokinase from concerted feedback inhibition by threonine and lysine. |
| Culture medium | LB5G medium PMB medium contains 80mM glucose,1.3mM threonine,and 0.2mM methionine,with the amino acids being added due to the auxotrophy of C.glutamicum ATCC 13287 for homoserine. |
| Carbon source | Glucose. |
| Growth rate | 0.4/h |
| Case-specific description | Intracellular flux distribution of lysine-producing C.glutamicum after 5.8h of cultivation |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 13287 |
| Genotype | This homoserine auxotrophic strain was derived from wild-type C.glutamicum ATCC 13032 by UV mutagenesis and selection.It overproduces lysine in the absence of threonine due to the release of aspartokinase from concerted feedback inhibition by threonine and lysine. |
| Culture medium | LB5G medium PMB medium contains 80mM glucose,1.3mM threonine,and 0.2mM methionine,with the amino acids being added due to the auxotrophy of C.glutamicum ATCC 13287 for homoserine. |
| Carbon source | Glucose. |
| Growth rate | 0.07/h |
| Case-specific description | Intracellular flux distribution of lysine-producing C.glutamicum after 6.9h of cultivation. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 13287 |
| Genotype | This homoserine auxotrophic strain was derived from wild-type C.glutamicum ATCC 13032 by UV mutagenesis and selection.It overproduces lysine in the absence of threonine due to the release of aspartokinase from concerted feedback inhibition by threonine and lysine. |
| Culture medium | LB5G medium PMB medium contains 80mM glucose,1.3mM threonine,and 0.2mM methionine,with the amino acids being added due to the auxotrophy of C.glutamicum ATCC 13287 for homoserine. |
| Carbon source | Glucose. |
| Growth rate | 0.07/h |
| Case-specific description | Intracellular flux distribution of lysine-producing C.glutamicum after 8.1h of cultivation. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 13287 |
| Genotype | This homoserine auxotrophic strain was derived from wild-type C.glutamicum ATCC 13032 by UV mutagenesis and selection.It overproduces lysine in the absence of threonine due to the release of aspartokinase from concerted feedback inhibition by threonine and lysine. |
| Culture medium | LB5G medium PMB medium contains 80mM glucose,1.3mM threonine,and 0.2mM methionine,with the amino acids being added due to the auxotrophy of C.glutamicum ATCC 13287 for homoserine. |
| Carbon source | Glucose. |
| Growth rate | 0.07/h |
| Case-specific description | Intracellular flux distribution of lysine-producing C.glutamicum after 9.2 h of cultivation. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 3.7 |
| lysine | 4.1 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 3.2 |
| lysine | 4.5 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.5 |
| lysine | 3.3 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2 |
| lysine | 2.5 |