Appl Environ Microbiol. 1997 Aug;63(8):3205-10. Reduction of aerobic acetate production by Escherichia coli. Farmer WR, Liao JC.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 701.35 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 24.4 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 726.4 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 26.4 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 736.05 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 26.2 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli JCL1225/pJF118EH |
| Genotype | |
| Culture medium | For strain construction,cells were grown in Luria-Bertani medium. For physiological characterizations, all strains were cultured in M9 minimal medium with 0.5% glucose. |
| Carbon source | D-Glucose |
| Growth rate | 0.52/h |
| Case-specific description | Relative flux distribution in a wild-type E.coli strain, in which the fluxes were calculated based on typical experimental yields of biomass, acetate, and CO2 and normalized by the glucose consumption. |
| Growth Condition | |
| Strains | Escherichia coli JCL1225/pPC201 |
| Genotype | |
| Culture medium | For strain construction,cells were grown in Luria-Bertani medium. For physiological characterizations, all strains were cultured in M9 minimal medium with 0.5% glucose. |
| Carbon source | D-Glucose |
| Growth rate | 0.57/h |
| Case-specific description | Relative flux distribution of an E.coli strain that overexpresses PPC.The glucose consumption and respiration fluxes were held at the same level as in case 1, while the PPC flux was specified at a value 8% higher than that calculated in case 1. The rest of the fluxes were calculated based on these three specified fluxes. |
| Growth Condition | |
| Strains | Escherichia coli JCL1535 |
| Genotype | |
| Culture medium | For strain construction,cells were grown in Luria-Bertani medium. For physiological characterizations, all strains were cultured in M9 minimal medium with 0.5% glucose. |
| Carbon source | D-Glucose |
| Growth rate | 0.52/h |
| Case-specific description | Relative flux distribution in an E.coli strain with deregulated glyoxylate bypass. The glucose consumption, respiration,and PPC fluxes were held at the same level as in case 1, while the glyoxylate bypass flux was specified at a value (0.017) such that the acetate flux was 50% of the control flux shown in case 1. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 9.44 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 8.89 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 10.56 |