J Bacteriol. 2001 Feb;183(4):1441-51. Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression. Gombert AK, Moreira dos Santos M, et al.
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| ATP production rate Maximum | -83.25 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | -99.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 38.9 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 32.4 | R02736+R01528 |
| ATP production rate Maximum | 297.25 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 130.75 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 155 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 88.4 | R02736+R01528 |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae CEN.PK113-7D |
| Genotype | (MATa MAL2-8cSUC2) |
| Culture medium | In all cases, cells from yeast-peptone-dextrose (YPD) plates were transferred to 500-ml baffled flasks containing 100 ml of the following medium: glucose, 10 g/liter; (NH4)2SO4, 7.5 g/liter; KH2PO4, 14.4 g/liter; MgSO4-7H2O, 0.48 g/liter;trace element solution, 2.0 ml/liter; vitamin solution, 1.0 ml/liter; pH was adjusted to 6.5. After about 24 h on a rotatory shaker at 30 Celsius and 150 /min, cells from this preculture were used to inoculate the bioreactor to an optical density at 600 nm (OD600) of 0.15 in the following medium: (NH4)2SO4, 5.0 g/liter; KH2PO4, 3.0 g/liter; MgSO4-7H2O, 0.5 g/liter; trace element solution, 1.0 ml/liter; vitamin solution, 1.0 ml/liter. In all the labeling experiments, 100% 1-13C-labeled glucose was used as the sole carbon source. In the batch cultures, 5 g/liter was used as the initial glucose concentration, and the cultivations were interrupted in the late exponential phase after at least 4 generation times, when cells were still growing at the maximum specific growth rate, mmax. The trace metal solution had the following composition (in grams per liter): EDTA, 15; ZnSO4-7H2O, 4.5; MnCl2-2H2O, 0.84; CoCl2-6H2O, 0.30;CuSO4-5H2O, 0.30; Na2MoO4-2H2O, 0.40; CaCl2-2H2O, 4.5; FeSO4-7H2O,3.0; H3BO3, 1.0; KI, 0.10. The composition of the vitamin solution was as follows (in grams per liter): D-biotin, 0.05; calcium pantothenate, 1.0; nicotinic acid, 1.0; myoinositol, 25.0; thiamine chloride hydrochloride, 1.0; pyridoxol hydrochloride, 1.0; p-aminobenzoic acid, 0.20. |
| Carbon source | D-Glucose |
| Growth rate | 0.37/h |
| Case-specific description | Fluxes in the central metabolism of S.cerevisiae correspond to respiro-fermentative metabolism (batch cultivation). |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae CEN.PK113-7D |
| Genotype | (MATa MAL2-8cSUC2) |
| Culture medium | In all cases, cells from yeast-peptone-dextrose (YPD) plates were transferred to 500-ml baffled flasks containing 100 ml of the following medium: glucose, 10 g/liter; (NH4)2SO4, 7.5 g/liter; KH2PO4, 14.4 g/liter; MgSO4-7H2O, 0.48 g/liter;trace element solution, 2.0 ml/liter; vitamin solution, 1.0 ml/liter; pH was adjusted to 6.5. After about 24 h on a rotatory shaker at 30 Celsius and 150 /min, cells from this preculture were used to inoculate the bioreactor to an optical density at 600 nm (OD600) of 0.15 in the following medium: (NH4)2SO4, 5.0 g/liter; KH2PO4, 3.0 g/liter; MgSO4-7H2O, 0.5 g/liter; trace element solution, 1.0 ml/liter; vitamin solution, 1.0 ml/liter. In all the labeling experiments, 100% 1-13C-labeled glucose was used as the sole carbon source. In the batch cultures, 5 g/liter was used as the initial glucose concentration, and the cultivations were interrupted in the late exponential phase after at least 4 generation times, when cells were still growing at the maximum specific growth rate, mmax. The trace metal solution had the following composition (in grams per liter): EDTA, 15; ZnSO4-7H2O, 4.5; MnCl2-2H2O, 0.84; CoCl2-6H2O, 0.30;CuSO4-5H2O, 0.30; Na2MoO4-2H2O, 0.40; CaCl2-2H2O, 4.5; FeSO4-7H2O,3.0; H3BO3, 1.0; KI, 0.10. The composition of the vitamin solution was as follows (in grams per liter): D-biotin, 0.05; calcium pantothenate, 1.0; nicotinic acid, 1.0; myoinositol, 25.0; thiamine chloride hydrochloride, 1.0; pyridoxol hydrochloride, 1.0; p-aminobenzoic acid, 0.20. |
| Carbon source | D-Glucose |
| Growth rate | |
| Case-specific description | Fluxes in the central metabolism of S.cerevisiae correspond to respiratory metabolism(chemostat). |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 15.9 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.17 |