Microb Cell Fact. 2014 Mar;13(1):40. Metabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains. Liu DF, Ai GM, Zheng QX, et al.
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ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | 655.8 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 69.6 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | 449.65 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 65.8 | R02736+R01528 |
Growth Condition | |
Strains | B.subtilis BSSA/pHCMC04/pDG148-stu |
Genotype | B.subtilis 1A474 carrying plasmids pHCMC04 and pDG148-stu |
Culture medium | Luria Bertani (LB) |
Carbon source | Glucose |
Growth rate | 0.434/h |
Case-specific description | Metabolic flux distribution in B.subtilis BSSA/pHCMC04/pDG148-stu during exponential growth phase. |
Growth Condition | |
Strains | B.subtilis BSSA/pSAAroA/pDGSAAroD |
Genotype | B.subtilis 1A474 carrying plasmids pSAAroA and pDGSAAroD |
Culture medium | Luria Bertani (LB) |
Carbon source | Glucose |
Growth rate | 0.332/h |
Case-specific description | Metabolic flux distribution in B.subtilis BSSA/pSAAroA/pDGSAAroD during exponential growth phase. |
Specific Rate | (mmol g-1 h-1) |
glucose | 5.199 |
shikimic acid | 0.101 |
3-dehydroshikimic acid | 0.132 |
Specific Rate | (mmol g-1 h-1) |
glucose | 4.666 |
shikimic acid | 0.159 |
3-dehydroshikimic acid | 0.048 |