Metab Eng. 1999 Jan;1(1):35-48. Response of the central metabolism in Corynebacterium glutamicum to the use of an NADH-dependent glutamate dehydrogenase. Marx A, Eikmanns BJ, Sahm H, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 445.25 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 152.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 743.55 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 52.8 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum MH20-22B |
| Genotype | delta gdh pEK1.9gdh-1 |
| Culture medium | continuous culture as described elsewhere (Marx et al.Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by NMR spectroscopy combined with metabolite balancing, 1996) except that the ammonium sulfate concentration was 600 mM,and isopropyl-beta-D-thiogalactoside (IPTG) (0.5 mM) and methionine S-sulfoximine (14.8 mM) were present. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | In vivo flux distribution in the central metabolism for C. glutamicum MH20-22B using its NADP-dependent glutamate dehydrogenase (homologous mutant MH20-22B delta gdh pEK1.9gdh-1) |
| Growth Condition | |
| Strains | Corynebacterium glutamicum MH20-22B |
| Genotype | delta dh pEKEXpgdh |
| Culture medium | continuous culture as described elsewhere (Marx et al.Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by NMR spectroscopy combined with metabolite balancing, 1996) except that the ammonium sulfate concentration was 600 mM,and isopropyl-beta-D-thiogalactoside (IPTG) (0.5 mM) and methionine S-sulfoximine (14.8 mM) were present. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | In vivo flux distribution in the central metabolism for C. glutamicum MH20-22B using the NAD-dependent glutamate dehydrogenase of Peptostreptococcus asaccharolyticus (heterologous mutant C.Glutamicum MH20-22B delta gdh pEKEXpgdh) |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.614 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.38 |