J Biotechnol. 2007 Apr;129(2):249-67. Metabolic flux analysis at ultra short time scale: isotopically non-stationary 13C labeling experiments. Noh K, Gronke K, Luo B, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli K12 |
| Genotype | Wild type |
| Culture medium | The Luria-Bertani (LB) medium contained 30% (v/v)glycerol.The precultivation medium contained 630mL fermentation medium: 8g/L glucose, 5g/L (NH4)2SO4, 3g/L KH2PO4, 1g/L NaCl, 0.3g/L MgSO4-2H2O, 0.112g/L FeSO4-7H2O, 0.75g/L thiamine, 0.15g/L 3,4-dihydroxybenzoate, 0.015g/L CaCl2-2H2O and trace elements: 0.75mg/L AlCl3-6H2O, 0.6mg/ CoCl2-6H2O, 2.5mg/L CuSO4-5H2O, 0.5mg/L H3BO3, 17.1mg/L MnSO4-1H2O, 3mg/L NaMoO4-2H2O, 1.7mg/L NiCl2-6H2O, 15mg/L ZnSO4-7H2O. The same medium was used for the precultures with the following change: 6g/L glucose.Fermentation was performed at 37 Celsius, pH 7, dissolvedoxygen (DO) 30% and a mixture of NH4/KOH was used for pH control. After glucose dropped to 1g/L,a glucose feed (60%, w/w) was started to maintain glucose concentration between 1 and 2g/L. |
| Carbon source | D-Glucose |
| Growth rate | |
| Case-specific description | Network model of the central metabolism of E.coli. |
| Specific Rate | (mmol g-1 h-1) |