Biotechnol Bioeng. 2004 Mar;85(5):497-505. Serial flux mapping of Corynebacterium glutamicum during fed-batch L-lysine production using the sensor reactor approach. Drysch A, El Massaoudi M, Wiechert W, et al.
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| ATP production rate Maximum | 918 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 700.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 399 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 312 | R02736+R01528 |
| ATP production rate Maximum | 655 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 507.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 377 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 318 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum MH-20-22B |
| Genotype | L-leucine auxotrophic strain,with feedbackresistant aspartate kinase and alteredL-lysine export carrier |
| Culture medium | The complex medium for the preculture contained yeast extract(10 g/L), peptone (10 g/L),glucose H2O (20 g/L),NaCl (2.5 g/L),and MgSO4 -7H2O (0.25 g/L).The synthetic medium used for the shake-flask preculture contained (NH4)2SO4 (46 g/L),urea (5 g/L),L-leucine (0.315 g/L),K2HPO4 (0.5 g/L),KH2PO4 (0.5 g/L),MgSO4-7H2O (0.285 g/L),CaCO3 (5 g/L),glucose-H2O (110 g/L),biotin (0.85 mg/L),FeSO4-7H2O (2.85 mg/L),MnSO4-H2O (1.165 mg/L),CuSO4-5H2O (0.0763 mg/L),ZnSO4-7H2O (0.63 mg/L),CoCl-6H2O (0.013 mg/L),NiCl-6H2O (4.25 ug/L), Na2MO4-2H2O(6.5 ug/L), KAlSO4-12H2O (0.019 mg/L),Na2SeO3-5H2O (1.93 ug/L),H3BO3 (5.0 ug/L),SrCl2-6H2O (5.0 ug/L),and BaCl2-2H2O (5.0 ug/L).The synthetic medium for the bioreactor cultivations was similar to the shake-flask medium except for (NH4)2SO4 (30 g/L),urea (6 g/L),L-leucine (0.8 g/L),Ca2Cl (0.05 g/L),dinitrilotetraacetic acid,EDTA (0.1 g/L ),and citric acid (0.1 g/L).No CaCO3 was added.The synthetic feed medium used for fed-batch mode was similar to the shake-flask medium except for L-leucine (0.5 g/L),K2HPO4 (0.2 g/L),KH2PO4 (0.2 g/L),Ca2Cl (0.05 g/L),EDTA (0.1 g/L),and citric acid (0.1 g/L).No CaCO3 was added. |
| Carbon source | Glucose |
| Growth rate | 0.2/h |
| Case-specific description | Metabolic flux distribution in nongrowing L-lysine producing C.glutamicum at the beginning of the L-leucine-limited production phase after a 24.5h process time. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum MH-20-22B |
| Genotype | L-leucine auxotrophic strain,with feedbackresistant aspartate kinase and alteredL-lysine export carrier |
| Culture medium | The complex medium for the preculture contained yeast extract(10 g/L), peptone (10 g/L),glucose H2O (20 g/L),NaCl (2.5 g/L),and MgSO4 -7H2O (0.25 g/L).The synthetic medium used for the shake-flask preculture contained (NH4)2SO4 (46 g/L),urea (5 g/L),L-leucine (0.315 g/L),K2HPO4 (0.5 g/L),KH2PO4 (0.5 g/L),MgSO4-7H2O (0.285 g/L),CaCO3 (5 g/L),glucose-H2O (110 g/L),biotin (0.85 mg/L),FeSO4-7H2O (2.85 mg/L),MnSO4-H2O (1.165 mg/L),CuSO4-5H2O (0.0763 mg/L),ZnSO4-7H2O (0.63 mg/L),CoCl-6H2O (0.013 mg/L),NiCl-6H2O (4.25 ug/L), Na2MO4-2H2O(6.5 ug/L), KAlSO4-12H2O (0.019 mg/L),Na2SeO3-5H2O (1.93 ug/L),H3BO3 (5.0 ug/L),SrCl2-6H2O (5.0 ug/L),and BaCl2-2H2O (5.0 ug/L).The synthetic medium for the bioreactor cultivations was similar to the shake-flask medium except for (NH4)2SO4 (30 g/L),urea (6 g/L),L-leucine (0.8 g/L),Ca2Cl (0.05 g/L),dinitrilotetraacetic acid,EDTA (0.1 g/L ),and citric acid (0.1 g/L).No CaCO3 was added.The synthetic feed medium used for fed-batch mode was similar to the shake-flask medium except for L-leucine (0.5 g/L),K2HPO4 (0.2 g/L),KH2PO4 (0.2 g/L),Ca2Cl (0.05 g/L),EDTA (0.1 g/L),and citric acid (0.1 g/L).No CaCO3 was added. |
| Carbon source | Glucose |
| Growth rate | 0.2/h |
| Case-specific description | Metabolic flux distribution in nongrowing L-lysine producing C.glutamicum at the beginning of theL-leucine-limited production phase after a 32h process time. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.05 |
| lysine | 0.31 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.75 |
| lysine | 0.28 |