Eur J Biochem. 2003 Sep;270(17):3525-42. Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry. Klapa MI, Aon JC, Stephanopoulos G.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 49.8 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 21799 |
| Genotype | |
| Culture medium | The seed culture medium was modified Luria–Bertani broth, containing: 5g/L glucose, 5g/L yeast extract, 10g/L tryptone,5g/L NaCl.The preculture and chemostat feed medium consisted of (per liter distilled water): 5g glucose, 50mg CaCl2, 400mg MgSO4-7H2O, 25mg FeSO4-7H2O, 0.1g NaCl, 10mL 100* mineral salts solution, 3g K2HPO4, 1g KH2PO4, 1g threonine, 0.3g methionine, 1g leucine, 1mg biotin, 1mg thiamine-HCl, 10mg pantothenic acid, 5g (NH4)2SO4 and 0.1uL antifoam.The 100* mineral salts solution consisted of (per liter distilled water): 200mg FeCl3-6H2O, 200mg MnSO4-H2O, 50mg ZnSO4-7H2O, 20mg CuCl2-2H2O, 20mg Na2B4O7-10H2O, 10mg (NH4)6Mo7O24-4H2O (pH was adjusted to 2.0 by addition of HCl to avoid precipitation). |
| Carbon source | Glucose |
| Growth rate | ./h |
| Case-specific description | The estimatednet flux distribution of C.glutamicum under glucose-limited growth in continuous culture(D=0.1/h). |
| Specific Rate | (mmol g-1 h-1) |
| acetate | 0 |
| glucose | -2.66 |
| lactate | 0 |
| lysine | 0.19 |
| trehalose | 0 |