FEMS Microbiol Lett. 2003 Mar;220(2):295-301. Analysis of metabolic and physiological responses to gnd knockout in Escherichia coli by using C-13 tracer experiment and enzyme activity measurement. Jiao Z, Baba T, Mori H, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Wild-type Escherichia coli BW25113 |
| Genotype | lacIq rrnBT14 delata lacZWJ16 hsdR514 delata araBADAH33 delta rhaBADLD78 |
| Culture medium | The minimal medium prepared with the exception for glucose concentration: 5 g/L for batch cultivation and 4 g/L for chemostat culture. Batch and chemostat cultures were conducted at 37 Celsius in a 2-l reactor with pH controlled at 7.0. |
| Carbon source | D-Glucose |
| Growth rate | 0.62/h |
| Case-specific description | Metabolic fux distribution in chemostat culture of E.coli BW25113 at a dilution rate of 0.2/h.Fluxes are given relative to the specifc glucose consumption rate (3.20 mmol/g/h). |
| Growth Condition | |
| Strains | Escherichia coli JWK 2011 |
| Genotype | gnd gene knock-out mutant |
| Culture medium | The minimal medium prepared with the exception for glucose concentration: 5 g/L for batch cultivation and 4 g/L for chemostat culture. Batch and chemostat cultures were conducted at 37 Celsius in a 2-l reactor with pH controlled at 7.0. |
| Carbon source | D-Glucose |
| Growth rate | 0.60/h |
| Case-specific description | Metabolic fux distribution in chemostat culture of gnd knock-out mutant at a dilution rate of 0.2/h.Fluxes are given relative to the specifc glucose consumption rate (3.75 mmol/g/h). |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 3.2 |
| acetate | 1.24 |
| CO2 | 4.19 |
| O2 | 6.5 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 3.75 |
| acetate | 2 |
| CO2 | 4.15 |
| O2 | 6.62 |