Eur J Biochem. 2001 Apr;268(8):2441-55. Application of MALDI-TOF MS to lysine-producing Corynebacterium glutamicum: a novel approach for metabolic flux analysis. Wittmann C, Heinzle E.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 396.45 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 142 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 21253 |
| Genotype | The strain is homoserine and leucine auxotroph, exhibits a concerted aspartate kinase inhibition caused by threonine and lysine and accumulates lysine under the limitation of threonine. |
| Culture medium | LB5G and PMB medium were used for precultures and main cultures, respectively. |
| Carbon source | Glucose |
| Growth rate | 0.53/h |
| Case-specific description | Distribution of carbon fluxes in the central metabolism of Corynebacterium glutamicum ATCC 21253 during the phase of lysine production in batch culture calculated from combined metabolite balancing and 13C tracer experiments with label measurement by MALDI-TOF MS. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.72 |