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Eur J Biochem. 2001 Apr;268(8):2441-55. Application of MALDI-TOF MS to lysine-producing Corynebacterium glutamicum: a novel approach for metabolic flux analysis. Wittmann C, Heinzle E.

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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
ATP production rate Minium 396.45 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 142 R02736+R01528
Growth Condition
Strains Corynebacterium glutamicum ATCC 21253
Genotype The strain is homoserine and leucine auxotroph, exhibits a concerted aspartate kinase inhibition caused by threonine and lysine and accumulates lysine under the limitation of threonine.
Culture medium LB5G and PMB medium were used for precultures and main cultures, respectively.
Carbon source Glucose
Growth rate 0.53/h
Case-specific description Distribution of carbon fluxes in the central metabolism of Corynebacterium glutamicum ATCC 21253 during the phase of lysine production in batch culture calculated from combined metabolite balancing and 13C tracer experiments with label measurement by MALDI-TOF MS.
Specific Rate (mmol g-1 h-1)
glucose 1.72