J Bacteriol. 2002 Jan;184(1):152-64. Metabolic flux responses to pyruvate kinase knockout in Escherichia coli. Emmerling M, Dauner M, Ponti A, et al.
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| ATP production rate Maximum | 1086 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 836 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 188 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 88 | R02736+R01528 |
| ATP production rate Maximum | 924.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 707 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 129 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 42 | R02736+R01528 |
| ATP production rate Maximum | 1012.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 747.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 108 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 2 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli JM101 |
| Genotype | F- traD36 lacIq delta(lacZ)M15 proA+ B+ supE thi delta(lac-proAB) |
| Culture medium | M9 minimal medium containing 5 g of glucose per liter, 42 mM Na2HPO4, 22 mM KH2PO4, 19 mM NH4Cl, and 9 mM NaCl. The following components were sterilized separately and then added (per liter of final medium): 1 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, and 1 ml of 1-mg thiamine HCl (filter sterilized). |
| Carbon source | Glucose |
| Growth rate | 0.09/h |
| Case-specific description | Metabolicflux distribution in chemostat cultures of E.coli JM101. The chemostats were operated under glucose limitation at a D of 0.09/h. |
| Growth Condition | |
| Strains | Escherichia coli JM101 |
| Genotype | F- traD36 lacIq delta(lacZ)M15 proA+ B+ supE thi delta(lac-proAB) |
| Culture medium | M9 minimal medium containing 5 g of glucose per liter, 42 mM Na2HPO4, 22 mM KH2PO4, 19 mM NH4Cl, and 9 mM NaCl. The following components were sterilized separately and then added (per liter of final medium): 1 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, and 1 ml of 1-mg thiamine HCl (filter sterilized). |
| Carbon source | Glucose |
| Growth rate | 0.04/h |
| Case-specific description | The chemostats were operated under glucose limitation at a D of 0.40/h. |
| Growth Condition | |
| Strains | Escherichia coli JM101 |
| Genotype | F- traD36 lacIq delta(lacZ)M15 proA+ B+ supE thi delta(lac-proAB) |
| Culture medium | M9 minimal medium containing 5 g of glucose per liter, 42 mM Na2HPO4, 22 mM KH2PO4, 19 mM NH4Cl, and 9 mM NaCl. The following components were sterilized separately and then added (per liter of final medium): 1 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, and 1 ml of 1-mg thiamine HCl (filter sterilized). |
| Carbon source | Ammonia |
| Growth rate | 0.09/h |
| Case-specific description | The chemostats were operated under ammonia limitation at a D of 0.09/h. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.4 |
| O2 | 4.6 |
| CO2 | 4.9 |
| acetaate | 0 |
| 2-Oxoglutarate | 0 |
| Pyruvate | 0 |
| Fumarate | 0 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.8 |
| O2 | 11.8 |
| CO2 | 12.4 |
| acetaate | 0 |
| 2-Oxoglutarate | 0 |
| Pyruvate | 0 |
| Fumarate | 0 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.2 |
| O2 | 5.1 |
| CO2 | 5.2 |
| acetaate | 1.3 |
| 2-Oxoglutarate | 0 |
| Pyruvate | 0.1 |
| Fumarate | 0 |