Yeast. 2004 Jul;21(9):769-79. Phenotypic characterization of glucose repression mutants of Saccharomyces cerevisiae using experiments with 13C-labelled glucose. Raghevendran V, Gombert AK, Christensen B, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 43.04 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 41.26 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 44.74 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 39.02 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 45.38 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 55 | R02736+R01528 |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae CEN.PK113-7D |
| Genotype | MATa MAL2-8c SUC2 |
| Culture medium | The seed medium consisted of the following components (in g/L): (NH4)2SO4 5.0 g/L, MgSO4 0.5 g/L, KH2PO4 3.0 g/L, trace element solution 1 ml,vitamin solution 1 ml, and glucose 5.0 g/L at pH 5.0 (Verduyn, 1992). Glucose and vitamin solutions were filter-sterilized and added aseptically before inoculation. A single colony from a fresh YPD plate culture was inoculated to the seed medium and incubated at 30 Celsius at 150 rpm in an orbital shaker for 24 h. The cultivation medium composition was the same as the inoculation medium, except that all components except for KH2PO4were scaled eight times to have a final glucose concentration of 40 g/L (Verduyn, 1992). |
| Carbon source | Glucose |
| Growth rate | 0.37/h |
| Case-specific description | reference strain(S.cerevisiae CEN.PK113-7D) |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae CEN.PK514-2B |
| Genotype | MATa reg1::loxp-Kan-loxP MAL2-8c SUC2 |
| Culture medium | The seed medium consisted of the following components (in g/L): (NH4)2SO4 5.0 g/L, MgSO4 0.5 g/L, KH2PO4 3.0 g/L, trace element solution 1 ml,vitamin solution 1 ml, and glucose 5.0 g/L at pH 5.0 (Verduyn, 1992). Glucose and vitamin solutions were filter-sterilized and added aseptically before inoculation. A single colony from a fresh YPD plate culture was inoculated to the seed medium and incubated at 30 Celsius at 150 rpm in an orbital shaker for 24 h. The cultivation medium composition was the same as the inoculation medium, except that all components except for KH2PO4were scaled eight times to have a final glucose concentration of 40 g/L (Verduyn, 1992). |
| Carbon source | Glucose |
| Growth rate | 0.40/h |
| Case-specific description | reg1 delta(S.cerevisiae CEN.PK514-2B) |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae T475 |
| Genotype | MATa mig1::MEL1 mig2::URA3 MEL1 MAL2-8c SUC2 |
| Culture medium | The seed medium consisted of the following components (in g/L): (NH4)2SO4 5.0 g/L, MgSO4 0.5 g/L, KH2PO4 3.0 g/L, trace element solution 1 ml,vitamin solution 1 ml, and glucose 5.0 g/L at pH 5.0 (Verduyn, 1992). Glucose and vitamin solutions were filter-sterilized and added aseptically before inoculation. A single colony from a fresh YPD plate culture was inoculated to the seed medium and incubated at 30 Celsius at 150 rpm in an orbital shaker for 24 h. The cultivation medium composition was the same as the inoculation medium, except that all components except for KH2PO4were scaled eight times to have a final glucose concentration of 40 g/L (Verduyn, 1992). |
| Carbon source | Glucose |
| Growth rate | 0.32/h |
| Case-specific description | mig1mig2 delta(S.cerevisiae T475) |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae T468 |
| Genotype | MATa mig1::MEL1 MAL2-8c SUC2 |
| Culture medium | The seed medium consisted of the following components (in g/L): (NH4)2SO4 5.0 g/L, MgSO4 0.5 g/L, KH2PO4 3.0 g/L, trace element solution 1 ml,vitamin solution 1 ml, and glucose 5.0 g/L at pH 5.0 (Verduyn, 1992). Glucose and vitamin solutions were filter-sterilized and added aseptically before inoculation. A single colony from a fresh YPD plate culture was inoculated to the seed medium and incubated at 30 Celsius at 150 rpm in an orbital shaker for 24 h. The cultivation medium composition was the same as the inoculation medium, except that all components except for KH2PO4were scaled eight times to have a final glucose concentration of 40 g/L (Verduyn, 1992). |
| Carbon source | Glucose |
| Growth rate | 0.37/h |
| Case-specific description | mig1 delta(S.cerevisiae T468) |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae CEN.PK513-3A |
| Genotype | MATa grr1::loxp-Kan-loxP MAL2-8c SUC2 |
| Culture medium | The seed medium consisted of the following components (in g/L): (NH4)2SO4 5.0 g/L, MgSO4 0.5 g/L, KH2PO4 3.0 g/L, trace element solution 1 ml,vitamin solution 1 ml, and glucose 5.0 g/L at pH 5.0 (Verduyn, 1992). Glucose and vitamin solutions were filter-sterilized and added aseptically before inoculation. A single colony from a fresh YPD plate culture was inoculated to the seed medium and incubated at 30 Celsius at 150 rpm in an orbital shaker for 24 h. The cultivation medium composition was the same as the inoculation medium, except that all components except for KH2PO4were scaled eight times to have a final glucose concentration of 40 g/L (Verduyn, 1992). |
| Carbon source | Glucose |
| Growth rate | 0.22/h |
| Case-specific description | grr1 delta(S.cerevisiae CEN.PK513-3A) |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae CEN.PK520-6B |
| Genotype | MATa hxk2::loxp-Kan-loxP MAL2-8c SUC2 |
| Culture medium | The seed medium consisted of the following components (in g/L): (NH4)2SO4 5.0 g/L, MgSO4 0.5 g/L, KH2PO4 3.0 g/L, trace element solution 1 ml,vitamin solution 1 ml, and glucose 5.0 g/L at pH 5.0 (Verduyn, 1992). Glucose and vitamin solutions were filter-sterilized and added aseptically before inoculation. A single colony from a fresh YPD plate culture was inoculated to the seed medium and incubated at 30 Celsius at 150 rpm in an orbital shaker for 24 h. The cultivation medium composition was the same as the inoculation medium, except that all components except for KH2PO4were scaled eight times to have a final glucose concentration of 40 g/L (Verduyn, 1992). |
| Carbon source | Glucose |
| Growth rate | 0.22/h |
| Case-specific description | hxk2 delta(S.cerevisiae CEN.PK520-6B) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |