Metab Eng. 2014 Jun 19;25C:30-37. A de novo NADPH generation pathway for improving lysine production of Corynebacterium glutamicum by rational design of the coenzyme specificity of glyceraldehyde 3-phosphate dehydrogenase. Bommareddy RR, Chen Z, Rappert S, et al.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 478.8 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 102 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 422.45 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 108 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ppc strain |
| Genotype | C.glutamicum ATCC 13032, with mutations of lysC and ppc |
| Culture medium | The medium used was a defined minimal media containing 30 g/L glucose, 20 g/L (NH4)2SO4,0.06 g/L CaCl2-2H2O, 0.5 g/L MgSO4-7H20, 2 g/L NaCl, 2 g/L KH2PO4, 2g/L K2HPO4, 15mg/L 3,4-dihydroxybenzoic acid, 0.5 mg/L biotin, 1.6 mg/L Thiamine-HCl, 3 mg/L nicotinic acid and 10 mL/L of 100 * trace elements.The fermentations were maintained at 30 celsius, pH 7.2, and aeration of 1 vvm. |
| Carbon source | Glucose. |
| Growth rate | |
| Case-specific description | Metabolicflux distributions in the C.glutamicum ppc strain during the exponential growth phase.(glucose uptake rates 5.4 mmol/g/h) |
| Growth Condition | |
| Strains | Corynebacterium glutamicum gapA M2 strain |
| Genotype | C. glutamicum ppc, with mutation of gapA |
| Culture medium | The medium used was a defined minimal media containing 30 g/L glucose, 20 g/L (NH4)2SO4,0.06 g/L CaCl2-2H2O, 0.5 g/L MgSO4-7H20, 2 g/L NaCl, 2 g/L KH2PO4, 2g/L K2HPO4, 15mg/L 3,4-dihydroxybenzoic acid, 0.5 mg/L biotin, 1.6 mg/L Thiamine-HCl, 3 mg/L nicotinic acid and 10 mL/L of 100 * trace elements.The fermentations were maintained at 30 celsius, pH 7.2, and aeration of 1 vvm. |
| Carbon source | Glucose. |
| Growth rate | |
| Case-specific description | Metabolicflux distributions in the C.glutamicum gapA M2 strain during the exponential growth phase.(glucose uptake rates 5.2 mmol/g/h) |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 5.4 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 5.2 |