Appl Microbiol Biotechnol. 2004 Jan;63(4):407-17. Metabolic flux analysis of pykF gene knockout Escherichia coli based on 13C-labeling experiments together with measurements of enzyme activities and intracellular metabolite concentrations. Al Zaid Siddiquee K, Arauzo-Bravo MJ, Shimizu K.
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| ATP production rate Maximum | 1001.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 776.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 158 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 68 | R02736+R01528 |
| ATP production rate Maximum | 1067.4 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 812.4 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 260 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 158 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli K-12 |
| Genotype | Wild type |
| Culture medium | A minimal medium consisting of 10 g glucose per liter, 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, and 40 mM (NH4)SO4, was used. For minimal medium, the following components were filter-sterilized separately and then added (per liter of final volume): 1 ml 1 M MgSO4, 1 ml 0.1 mM CaCl2, 1 ml vitamin B1 (1 mg/l stock) and 10 ml trace element solution containing (per liter): 0.55 g CaCl2, 1 g FeCl3, 0.1 g MnCl2-4H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-2H2O, and 0.06 g Na2MoO4-2H2O. |
| Carbon source | D-Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux distribution of wild type at dilution rate of 0.1/h. |
| Growth Condition | |
| Strains | Escherichia coli JWK1666 |
| Genotype | the PykF(pykF-) knockout mutant |
| Culture medium | A minimal medium consisting of 10 g glucose per liter, 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, and 40 mM (NH4)SO4, was used. For minimal medium, the following components were filter-sterilized separately and then added (per liter of final volume): 1 ml 1 M MgSO4, 1 ml 0.1 mM CaCl2, 1 ml vitamin B1 (1 mg/l stock) and 10 ml trace element solution containing (per liter): 0.55 g CaCl2, 1 g FeCl3, 0.1 g MnCl2-4H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-2H2O, and 0.06 g Na2MoO4-2H2O. |
| Carbon source | D-Glucose |
| Growth rate | 0.5/h |
| Case-specific description | Metabolic flux distribution of pykF- mutant at dilution rate of 0.1/h. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.81 |
| CO2 | 3.7 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 6.94 |
| CO2 | 16.8 |