J Biol Chem. 2004 Feb;279(8):6613-9. The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli. Sauer U, Canonaco F, Heri S, et al.
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| ATP production rate Maximum | 518 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 425.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 89 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 52 | R02736+R01528 |
| ATP production rate Maximum | 512.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 440 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 118 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 89 | R02736+R01528 |
| ATP production rate Maximum | 962.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 777.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 74 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| ATP production rate Maximum | 1063 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 918 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 246 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 188 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli MG1655 |
| Genotype | Wild type |
| Culture medium | M9 minimal medium, supplemented with 5.0 g/liter of either glucose, acetate,glycerol, or gluconate as the sole carbon source. Unless indicated otherwise, batch cultures were grown in 500-ml baffled shake flasks with maximally 50 ml of medium at 37 Celsius on a gyratory shaker at 200 rpm. |
| Carbon source | Glucose |
| Growth rate | 0.67/h |
| Case-specific description | The carbon flux distribution in central metabolism of E.coli wild-type during maximum exponential growth on glucose. |
| Growth Condition | |
| Strains | Escherichia coli UdhA-PntAB |
| Genotype | delta audhA delta pntAB |
| Culture medium | M9 minimal medium, supplemented with 5.0 g/liter of either glucose, acetate,glycerol, or gluconate as the sole carbon source. Unless indicated otherwise, batch cultures were grown in 500-ml baffled shake flasks with maximally 50 ml of medium at 37 Celsius on a gyratory shaker at 200 rpm. |
| Carbon source | Glucose |
| Growth rate | 0.52/h |
| Case-specific description | The carbon flux distribution in central metabolism of the E.coli UdhA-PntAB mutant during maximum exponential growth on glucose. |
| Growth Condition | |
| Strains | Escherichia coli Zwf-EDP |
| Genotype | delta(zwf edd eda) |
| Culture medium | M9 minimal medium, supplemented with 5.0 g/liter of either glucose, acetate,glycerol, or gluconate as the sole carbon source. Unless indicated otherwise, batch cultures were grown in 500-ml baffled shake flasks with maximally 50 ml of medium at 37 Celsius on a gyratory shaker at 200 rpm. |
| Carbon source | Glucose |
| Growth rate | 0.52/h |
| Case-specific description | The carbon flux distribution in central metabolism of the E.coli Zwf-EDP during maximum exponential growth on glucose. |
| Growth Condition | |
| Strains | Escherichia coli Pgi |
| Genotype | delta pgi |
| Culture medium | M9 minimal medium, supplemented with 5.0 g/liter of either glucose, acetate,glycerol, or gluconate as the sole carbon source. Unless indicated otherwise, batch cultures were grown in 500-ml baffled shake flasks with maximally 50 ml of medium at 37 Celsius on a gyratory shaker at 200 rpm. |
| Carbon source | Glucose |
| Growth rate | 0.18/h |
| Case-specific description | The carbon flux distribution in central metabolism of the E.coli Pgi mutants during maximum exponential growth on glucose. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 7.5 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 8.4 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 8.7 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.9 |