Metab Eng. 2003 Apr;5(2):96-107. Production process monitoring by serial mapping of microbial carbon flux distributions using a novel Sensor Reactor approach: II--(13)C-labeling-based metabolic flux analysis and L-lysine production. Drysch A, El Massaoudi M, Mack C, et al.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum MH20-22B |
| Genotype | derived from C.glutamicum ATCC 13032 (wild-type) by undirected mutagenesis |
| Culture medium | For large-scale batch conditions,cultivation was carried out in a production reactor (total reactor volume 300 L) which was connected to a small Sensor Reactor (1 L). The detailed principles of the approach as well as the medium compositions used are described by El Massaoudi et al. |
| Carbon source | Glucose |
| Growth rate | 0.2/h |
| Case-specific description | Distribution of carbon fluxes in the central metabolism of C.glutamicum during exponential growth in three parallel production and Sensor Reactor fermentations(phase 1). |
| Growth Condition | |
| Strains | Corynebacterium glutamicum MH20-22B |
| Genotype | derived from C.glutamicum ATCC 13032 (wild-type) by undirected mutagenesis |
| Culture medium | For large-scale batch conditions,cultivation was carried out in a production reactor (total reactor volume 300 L) which was connected to a small Sensor Reactor (1 L). The detailed principles of the approach as well as the medium compositions used are described by El Massaoudi et al. |
| Carbon source | Glucose |
| Growth rate | 0.2/h |
| Case-specific description | Distribution of carbon fluxes in the central metabolism of C.glutamicum during exponential growth in three parallel production and Sensor Reactor fermentations(phase 2). |
| Growth Condition | |
| Strains | Corynebacterium glutamicum MH20-22B |
| Genotype | derived from C.glutamicum ATCC 13032 (wild-type) by undirected mutagenesis |
| Culture medium | For large-scale batch conditions,cultivation was carried out in a production reactor (total reactor volume 300 L) which was connected to a small Sensor Reactor (1 L). The detailed principles of the approach as well as the medium compositions used are described by El Massaoudi et al. |
| Carbon source | Glucose |
| Growth rate | 0.2/h |
| Case-specific description | Distribution of carbon fluxes in the central metabolism of C.glutamicum during exponential growth in three parallel production and Sensor Reactor fermentations(phase 3). |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 3.13 |
| lysine | 0.13 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 3.23 |
| lysine | 0.27 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 3.19 |
| lysine | 0.41 |