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Metab Eng. 2003 Apr;5(2):96-107. Production process monitoring by serial mapping of microbial carbon flux distributions using a novel Sensor Reactor approach: II--(13)C-labeling-based metabolic flux analysis and L-lysine production. Drysch A, El Massaoudi M, Mack C, et al.



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ATP production rate Maximum 1227.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 897.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 208 R02736+R01528+R00267+R00214
NADPH production rate Minium 76 R02736+R01528
ATP production rate Maximum 1127.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 835 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 177 R02736+R01528+R00267+R00214
NADPH production rate Minium 60 R02736+R01528
ATP production rate Maximum 1018 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 773 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 166 R02736+R01528+R00267+R00214
NADPH production rate Minium 68 R02736+R01528
Growth Condition
Strains Corynebacterium glutamicum MH20-22B
Genotype derived from C.glutamicum ATCC 13032 (wild-type) by undirected mutagenesis
Culture medium For large-scale batch conditions,cultivation was carried out in a production reactor (total reactor volume 300 L) which was connected to a small Sensor Reactor (1 L). The detailed principles of the approach as well as the medium compositions used are described by El Massaoudi et al.
Carbon source Glucose
Growth rate 0.2/h
Case-specific description Distribution of carbon fluxes in the central metabolism of C.glutamicum during exponential growth in three parallel production and Sensor Reactor fermentations(phase 1).
Growth Condition
Strains Corynebacterium glutamicum MH20-22B
Genotype derived from C.glutamicum ATCC 13032 (wild-type) by undirected mutagenesis
Culture medium For large-scale batch conditions,cultivation was carried out in a production reactor (total reactor volume 300 L) which was connected to a small Sensor Reactor (1 L). The detailed principles of the approach as well as the medium compositions used are described by El Massaoudi et al.
Carbon source Glucose
Growth rate 0.2/h
Case-specific description Distribution of carbon fluxes in the central metabolism of C.glutamicum during exponential growth in three parallel production and Sensor Reactor fermentations(phase 2).
Growth Condition
Strains Corynebacterium glutamicum MH20-22B
Genotype derived from C.glutamicum ATCC 13032 (wild-type) by undirected mutagenesis
Culture medium For large-scale batch conditions,cultivation was carried out in a production reactor (total reactor volume 300 L) which was connected to a small Sensor Reactor (1 L). The detailed principles of the approach as well as the medium compositions used are described by El Massaoudi et al.
Carbon source Glucose
Growth rate 0.2/h
Case-specific description Distribution of carbon fluxes in the central metabolism of C.glutamicum during exponential growth in three parallel production and Sensor Reactor fermentations(phase 3).
Specific Rate (mmol g-1 h-1)
Glucose 3.13
lysine 0.13
Specific Rate (mmol g-1 h-1)
Glucose 3.23
lysine 0.27
Specific Rate (mmol g-1 h-1)
Glucose 3.19
lysine 0.41
Source