Metab Eng. 2003 Apr;5(2):96-107. Production process monitoring by serial mapping of microbial carbon flux distributions using a novel Sensor Reactor approach: II--(13)C-labeling-based metabolic flux analysis and L-lysine production. Drysch A, El Massaoudi M, Mack C, et al.
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ATP production rate Maximum | 1227.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | 897.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 208 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 76 | R02736+R01528 |
ATP production rate Maximum | 1127.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | 835 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 177 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 60 | R02736+R01528 |
ATP production rate Maximum | 1018 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | 773 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 166 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 68 | R02736+R01528 |
Growth Condition | |
Strains | Corynebacterium glutamicum MH20-22B |
Genotype | derived from C.glutamicum ATCC 13032 (wild-type) by undirected mutagenesis |
Culture medium | For large-scale batch conditions,cultivation was carried out in a production reactor (total reactor volume 300 L) which was connected to a small Sensor Reactor (1 L). The detailed principles of the approach as well as the medium compositions used are described by El Massaoudi et al. |
Carbon source | Glucose |
Growth rate | 0.2/h |
Case-specific description | Distribution of carbon fluxes in the central metabolism of C.glutamicum during exponential growth in three parallel production and Sensor Reactor fermentations(phase 1). |
Growth Condition | |
Strains | Corynebacterium glutamicum MH20-22B |
Genotype | derived from C.glutamicum ATCC 13032 (wild-type) by undirected mutagenesis |
Culture medium | For large-scale batch conditions,cultivation was carried out in a production reactor (total reactor volume 300 L) which was connected to a small Sensor Reactor (1 L). The detailed principles of the approach as well as the medium compositions used are described by El Massaoudi et al. |
Carbon source | Glucose |
Growth rate | 0.2/h |
Case-specific description | Distribution of carbon fluxes in the central metabolism of C.glutamicum during exponential growth in three parallel production and Sensor Reactor fermentations(phase 2). |
Growth Condition | |
Strains | Corynebacterium glutamicum MH20-22B |
Genotype | derived from C.glutamicum ATCC 13032 (wild-type) by undirected mutagenesis |
Culture medium | For large-scale batch conditions,cultivation was carried out in a production reactor (total reactor volume 300 L) which was connected to a small Sensor Reactor (1 L). The detailed principles of the approach as well as the medium compositions used are described by El Massaoudi et al. |
Carbon source | Glucose |
Growth rate | 0.2/h |
Case-specific description | Distribution of carbon fluxes in the central metabolism of C.glutamicum during exponential growth in three parallel production and Sensor Reactor fermentations(phase 3). |
Specific Rate | (mmol g-1 h-1) |
Glucose | 3.13 |
lysine | 0.13 |
Specific Rate | (mmol g-1 h-1) |
Glucose | 3.23 |
lysine | 0.27 |
Specific Rate | (mmol g-1 h-1) |
Glucose | 3.19 |
lysine | 0.41 |