Appl Environ Microbiol. 2011 Sep;77(18):6644-52. Comparative 13C metabolic flux analysis of pyruvate dehydrogenase complex-deficient, L-valine-producing Corynebacterium glutamicum. Bartek T, Blombach B, Lang S, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 3.14 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.82 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 1.4 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 1.66 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.58 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum |
| Genotype | Wild type. |
| Culture medium | LB medium for the first and second preculture. CGXII medium contained 222mM glucose (40 g/L) and 100mM acetate (6 g/L) as carbon sources.Morpholinepropanesulfonic acid (MOPS) buffer(42 g/L) was used in the second preculture. |
| Carbon source | Glucose |
| Growth rate | 0.43/h |
| Case-specific description | Quantification of intracellular fluxes in wild-type C.glutamicum. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum delta aceE |
| Genotype | delta aceE |
| Culture medium | LB medium for the first and second preculture. CGXII medium contained 222mM glucose (40 g/L) and 100mM acetate (6 g/L) as carbon sources.Morpholinepropanesulfonic acid (MOPS) buffer(42 g/L) was used in the second preculture. |
| Carbon source | Glucose |
| Growth rate | 0.43/h |
| Case-specific description | Quantification of intracellular fluxes in C.glutamicum delta aceE. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum delta ace(pJC4ilvBNCE) |
| Genotype | delta ace(pJC4ilvBNCE) |
| Culture medium | LB medium for the first and second preculture. CGXII medium contained 222mM glucose (40 g/L) and 100mM acetate (6 g/L) as carbon sources.Morpholinepropanesulfonic acid (MOPS) buffer(42 g/L) was used in the second preculture. |
| Carbon source | Glucose |
| Growth rate | 0.43/h |
| Case-specific description | Quantification of intracellular fluxes in C.glutamicum delta ace(pJC4ilvBNCE). |
| Growth Condition | |
| Strains | Corynebacterium glutamicum delta ace delta pqo pJC4ilvBNCE |
| Genotype | delta ace delta pqo pJC4ilvBNCE |
| Culture medium | LB medium for the first and second preculture. CGXII medium contained 222mM glucose (40 g/L) and 100mM acetate (6 g/L) as carbon sources.Morpholinepropanesulfonic acid (MOPS) buffer(42 g/L) was used in the second preculture. |
| Carbon source | Glucose |
| Growth rate | 0.43/h |
| Case-specific description | Quantification of intracellular fluxes in C.glutamicum delta ace delta pqo pJC4ilvBNCE. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum delta ace delta pqo(pJC4ilvBNCE)(pBB1pntAB) |
| Genotype | delta ace delta pqo(pJC4ilvBNCE)(pBB1pntAB) |
| Culture medium | LB medium for the first and second preculture. CGXII medium contained 222mM glucose (40 g/L) and 100mM acetate (6 g/L) as carbon sources.Morpholinepropanesulfonic acid (MOPS) buffer(42 g/L) was used in the second preculture. |
| Carbon source | Glucose |
| Growth rate | 0.43/h |
| Case-specific description | Quantification of intracellular fluxes in C.glutamicum delta ace delta pqo(pJC4ilvBNCE)(pBB1pntAB). |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.28 |
| valine | N/A |
| alanine | N/A |
| ketoisovalerate | N/A |
| CO2 | 1.57 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.53 |
| valine | 0.1 |
| alanine | 0.26 |
| ketoisovalerate | 0.04 |
| CO2 | 0.55 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.7 |
| valine | 0.39 |
| alanine | 0.01 |
| ketoisovalerate | N/A |
| CO2 | 0.39 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.73 |
| valine | 0.58 |
| alanine | 0.01 |
| ketoisovalerate | 0.01 |
| CO2 | 1.41 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.51 |
| valine | 0.46 |
| alanine | 0.01 |
| ketoisovalerate | 0 |
| CO2 | 0.75 |