Microb Cell Fact. 2012 Oct;11:138. Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine. Peifer S, Barduhn T, Zimmet S, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 148.2 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 89.8 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 265.1 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 195.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 186.3 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 58.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 305 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 196 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 13032 |
| Genotype | Wild type. |
| Culture medium | The first precultures were grown on complex media (pH6.8) containing per liter: 10.0g glucose,2.5g NaCl,2.0g urea,5.0g yeast extract,5.0g beef extract,5.0g polypeptone and 20.0g casamino acids.For agar plates,the complex medium was supplemented with 20.0g/L agar. Second precultures and main cultures were grown on minimal medium (pH 7.2) containing per liter: 15.0g glucose,4.0g KH2PO4,16.0g Na2HPO4,500mg MgCl2-6H2O,300mg 3,4-dihydroxibenzoic acid,100mg CaCl2-2H2O,100ug cyanocobalamin,750ug thiamine,4ug pyridoxal phosphate,100ug biotin,400ug calcium pantothenate,2ug folic acid,400ug nicotinic acid,200ug 4-aminobenzoic acid,400ug pyridoxine-HCl,2mg inositol,10mg FeCl2-4H2O,1mg ZnCl2,100ug CuCl2,20ug NiCl2-6H2O,20ug Na2MoO4-2H2O,500ug boric acid,100ug KI, 100ug CoCl2-6H2O and 10 mg MnCl2-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.443/h |
| Case-specific description | Intracellular carbon flux distributions with corresponding standard deviations of the central metabolism of the wild type C.glutamicum ATCC 13032. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum delta pgi |
| Genotype | deletion of the pgi gene(NCgl0817) |
| Culture medium | The first precultures were grown on complex media (pH6.8) containing per liter: 10.0g glucose,2.5g NaCl,2.0g urea,5.0g yeast extract,5.0g beef extract,5.0g polypeptone and 20.0g casamino acids.For agar plates,the complex medium was supplemented with 20.0g/L agar. Second precultures and main cultures were grown on minimal medium (pH 7.2) containing per liter: 15.0g glucose,4.0g KH2PO4,16.0g Na2HPO4,500mg MgCl2-6H2O,300mg 3,4-dihydroxibenzoic acid,100mg CaCl2-2H2O,100ug cyanocobalamin,750ug thiamine,4ug pyridoxal phosphate,100ug biotin,400ug calcium pantothenate,2ug folic acid,400ug nicotinic acid,200ug 4-aminobenzoic acid,400ug pyridoxine-HCl,2mg inositol,10mg FeCl2-4H2O,1mg ZnCl2,100ug CuCl2,20ug NiCl2-6H2O,20ug Na2MoO4-2H2O,500ug boric acid,100ug KI, 100ug CoCl2-6H2O and 10 mg MnCl2-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.376/h |
| Case-specific description | Intracellular carbon flux distributions with corresponding standard deviations of the central metabolism of mutant strains C.glutamicum delta pgi. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum delta purA delta guaB2 |
| Genotype | double deletion of purA and guaB2 genes |
| Culture medium | The first precultures were grown on complex media (pH6.8) containing per liter: 10.0g glucose,2.5g NaCl,2.0g urea,5.0g yeast extract,5.0g beef extract,5.0g polypeptone and 20.0g casamino acids.For agar plates,the complex medium was supplemented with 20.0g/L agar. Second precultures and main cultures were grown on minimal medium (pH 7.2) containing per liter: 15.0g glucose,4.0g KH2PO4,16.0g Na2HPO4,500mg MgCl2-6H2O,300mg 3,4-dihydroxibenzoic acid,100mg CaCl2-2H2O,100ug cyanocobalamin,750ug thiamine,4ug pyridoxal phosphate,100ug biotin,400ug calcium pantothenate,2ug folic acid,400ug nicotinic acid,200ug 4-aminobenzoic acid,400ug pyridoxine-HCl,2mg inositol,10mg FeCl2-4H2O,1mg ZnCl2,100ug CuCl2,20ug NiCl2-6H2O,20ug Na2MoO4-2H2O,500ug boric acid,100ug KI, 100ug CoCl2-6H2O and 10 mg MnCl2-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.317/h |
| Case-specific description | Intracellular carbon flux distributions with corresponding standard deviations of the central metabolism of C.glutamicum delta purA delta guaB2. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum delta purA delta guaB2 purFK348Q delta pgi |
| Genotype | deletion of purA,guaB2 and pgi genes and allelic amino acid substitution K348Q in the purF gene |
| Culture medium | The first precultures were grown on complex media (pH6.8) containing per liter: 10.0g glucose,2.5g NaCl,2.0g urea,5.0g yeast extract,5.0g beef extract,5.0g polypeptone and 20.0g casamino acids.For agar plates,the complex medium was supplemented with 20.0g/L agar. Second precultures and main cultures were grown on minimal medium (pH 7.2) containing per liter: 15.0g glucose,4.0g KH2PO4,16.0g Na2HPO4,500mg MgCl2-6H2O,300mg 3,4-dihydroxibenzoic acid,100mg CaCl2-2H2O,100ug cyanocobalamin,750ug thiamine,4ug pyridoxal phosphate,100ug biotin,400ug calcium pantothenate,2ug folic acid,400ug nicotinic acid,200ug 4-aminobenzoic acid,400ug pyridoxine-HCl,2mg inositol,10mg FeCl2-4H2O,1mg ZnCl2,100ug CuCl2,20ug NiCl2-6H2O,20ug Na2MoO4-2H2O,500ug boric acid,100ug KI, 100ug CoCl2-6H2O and 10 mg MnCl2-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.146/h |
| Case-specific description | Intracellular carbon flux distributions with corresponding standard deviations of the central metabolism of C.glutamicum delta purA delta guaB2 purFK348Q delta pgi. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.67 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.18 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.24 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.29 |