J Biotechnol. 2003 Mar;101(2):101-17. Metabolic flux analysis of Escherichia coli K12 grown on 13C-labeled acetate and glucose using GC-MS and powerful flux calculation method. Zhao J, Shimizu K.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 470.65 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 86 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 510.35 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 56.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 60.85 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 3.14 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 62.03 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 3.4 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli K12 |
| Genotype | |
| Culture medium | The minimal medium (E.coli K12) contained 4 g of glucose per liter, 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, and 30 mM (NH4)2SO4. The following components were sterilized separately and then added (per liter of final medium): 1 ml of 1 M MgSO4, 1 ml of 0.1 mM CaCl2, 1 ml of 1 mg of vitamin B1per liter (filter sterilized), and 10 ml of trace element solution containing (per liter) 0.55 g of CaCl2, 1 g of FeCl3, 0.1 g of MnCl2-4H2O, 0.17 g of ZnCl2, 0.043 g of CuCl2-2H2O, 0.06 g of CoCl2-6H2O,and 0.06 g of Na2MoO4-2H2O.The pH of the culture was maintained at 7.0 by automatic addition of 2.0 M HCl or 2.0 N NaOH with a pH controller. For labeling experiment,the feed medium containing 4 g/L unlabeled glucose was then replaced by the mixture of 0.3 g uniformly labeled glucose [U-13C], 0.3 g first carbon labeled glucose [1-13C] and 3.4 g of naturally labeled glucose per liter. |
| Carbon source | D-Glucose |
| Growth rate | 0.22/h |
| Case-specific description | Net flux distribution in glucose metabolism of E.coli K12 in chemostat cultures at D of 0.22/h. |
| Growth Condition | |
| Strains | Escherichia coli K12 |
| Genotype | |
| Culture medium | The minimal medium (E.coli K12) contained 4 g of glucose per liter, 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, and 30 mM (NH4)2SO4. The following components were sterilized separately and then added (per liter of final medium): 1 ml of 1 M MgSO4, 1 ml of 0.1 mM CaCl2, 1 ml of 1 mg of vitamin B1per liter (filter sterilized), and 10 ml of trace element solution containing (per liter) 0.55 g of CaCl2, 1 g of FeCl3, 0.1 g of MnCl2-4H2O, 0.17 g of ZnCl2, 0.043 g of CuCl2-2H2O, 0.06 g of CoCl2-6H2O,and 0.06 g of Na2MoO4-2H2O.The pH of the culture was maintained at 7.0 by automatic addition of 2.0 M HCl or 2.0 N NaOH with a pH controller. For labeling experiment,the feed medium containing 4 g/L unlabeled glucose was then replaced by the mixture of 0.3 g uniformly labeled glucose [U-13C], 0.3 g first carbon labeled glucose [1-13C] and 3.4 g of naturally labeled glucose per liter. |
| Carbon source | D-Glucose |
| Growth rate | 0.11/h |
| Case-specific description | Net flux distribution in glucose metabolism of E.coli K12 in chemostat cultures at D of 0.11/h. |
| Growth Condition | |
| Strains | Escherichia coli K12 |
| Genotype | |
| Culture medium | The minimal medium (E.coli K12) contained 2 g of sodium acetate per liter, 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, and 30 mM (NH4)2SO4. The following components were sterilized separately and then added (per liter of final medium): 1 ml of 1 M MgSO4, 1 ml of 0.1 mM CaCl2, 1 ml of 1 mg of vitamin B1per liter (filter sterilized), and 10 ml of trace element solution containing (per liter) 0.55 g of CaCl2, 1 g of FeCl3, 0.1 g of MnCl2-4H2O, 0.17 g of ZnCl2, 0.043 g of CuCl2-2H2O, 0.06 g of CoCl2-6H2O,and 0.06 g of Na2MoO4-2H2O.The pH of the culture was maintained at 7.0 by automatic addition of 2.0 M HCl or 2.0 N NaOH with a pH controller. For labeling experiment,the feed medium containing 2 g of unlabeled sodium acetate per liter was then replaced by an identical medium containing 1.8 g of sodium acetate labeled by natural abundance per liter and 0.2 g of [2-13C] sodium acetate per liter. |
| Carbon source | Acetate |
| Growth rate | 0.22/h |
| Case-specific description | Net flux distribution in acetate metabolism of E.coli K12 in chemostat cultures at D of 0.22/h. |
| Growth Condition | |
| Strains | Escherichia coli K12 |
| Genotype | |
| Culture medium | The minimal medium (E.coli K12) contained 2 g of sodium acetate per liter, 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, and 30 mM (NH4)2SO4. The following components were sterilized separately and then added (per liter of final medium): 1 ml of 1 M MgSO4, 1 ml of 0.1 mM CaCl2, 1 ml of 1 mg of vitamin B1per liter (filter sterilized), and 10 ml of trace element solution containing (per liter) 0.55 g of CaCl2, 1 g of FeCl3, 0.1 g of MnCl2-4H2O, 0.17 g of ZnCl2, 0.043 g of CuCl2-2H2O, 0.06 g of CoCl2-6H2O,and 0.06 g of Na2MoO4-2H2O.The pH of the culture was maintained at 7.0 by automatic addition of 2.0 M HCl or 2.0 N NaOH with a pH controller. For labeling experiment,the feed medium containing 2 g of unlabeled sodium acetate per liter was then replaced by an identical medium containing 1.8 g of sodium acetate labeled by natural abundance per liter and 0.2 g of [2-13C] sodium acetate per liter. |
| Carbon source | Acetate |
| Growth rate | 0.11/h |
| Case-specific description | Net flux distribution in acetate metabolism of E.coli K12 in chemostat cultures at D of 0.11/h. |
| Specific Rate | (mmol g-1 h-1) |
| acetate | 14.43 |
| CO2 | 17.32 |
| glucose | N/A |
| Specific Rate | (mmol g-1 h-1) |
| acetate | 7.18 |
| CO2 | 8.76 |
| glucose | N/A |
| Specific Rate | (mmol g-1 h-1) |
| acetate | 0.97 |
| CO2 | 11.37 |
| glucose | 5.92 |
| Specific Rate | (mmol g-1 h-1) |
| acetate | 0.38 |
| CO2 | 5.25 |
| glucose | 2.78 |