Microb Cell Fact. 2007 Jun;6:19. Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis. Shirai T, Fujimura K, Furusawa C, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 80 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 52 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 38 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum AJ-1511 (ATCC13869) |
| Genotype | |
| Culture medium | The same medium as that employed in our previous study was used for the preculture of the microorganism (per liter deionized water): 40g of glucose,30g of (NH4)2SO4, 3.0g of Na2HPO4, 6.0g of KH2PO4, 2.0g of NaCl, 84mg of CaCl2, 3.9mg of FeCl3, 0.9mg of ZnSO4-7H2O, 0.3mg of CuCl2-H2O, 5.56mg of MnSO4-5H2O, 0.1mg of (NH4)6MO7O24-4H2O, 0.3mg of Na2B4O7-10H2O, 0.4g of MgSO4-7H2O, 40mg of FeSO4-7H2O, 500ug of vitamin B1-HCl, 0.1g of EDTA,and 10ug of biotin. |
| Carbon source | Glucose |
| Growth rate | 0.25/h |
| Case-specific description | Metabolic fluxes in growth phase of C.glutamicum |
| Growth Condition | |
| Strains | Corynebacterium glutamicum AJ-1511 (ATCC13869) |
| Genotype | |
| Culture medium | The same medium as that employed in our previous study was used for the preculture of the microorganism (per liter deionized water): 40g of glucose,30g of (NH4)2SO4, 3.0g of Na2HPO4, 6.0g of KH2PO4, 2.0g of NaCl, 84mg of CaCl2, 3.9mg of FeCl3, 0.9mg of ZnSO4-7H2O, 0.3mg of CuCl2-H2O, 5.56mg of MnSO4-5H2O, 0.1mg of (NH4)6MO7O24-4H2O, 0.3mg of Na2B4O7-10H2O, 0.4g of MgSO4-7H2O, 40mg of FeSO4-7H2O, 500ug of vitamin B1-HCl, 0.1g of EDTA,and 10ug of biotin. |
| Carbon source | Glucose |
| Growth rate | 0.04/h |
| Case-specific description | Metabolic fluxes in glutamate production phase of C. glutamicum, where glutamate flux was 20 with respect to glucose uptake of 100. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum AJ-1511 (ATCC13869) |
| Genotype | |
| Culture medium | The same medium as that employed in our previous study was used for the preculture of the microorganism (per liter deionized water): 40g of glucose,30g of (NH4)2SO4, 3.0g of Na2HPO4, 6.0g of KH2PO4, 2.0g of NaCl, 84mg of CaCl2, 3.9mg of FeCl3, 0.9mg of ZnSO4-7H2O, 0.3mg of CuCl2-H2O, 5.56mg of MnSO4-5H2O, 0.1mg of (NH4)6MO7O24-4H2O, 0.3mg of Na2B4O7-10H2O, 0.4g of MgSO4-7H2O, 40mg of FeSO4-7H2O, 500ug of vitamin B1-HCl, 0.1g of EDTA,and 10ug of biotin. |
| Carbon source | Glucose |
| Growth rate | 0.01/h |
| Case-specific description | Metabolic fluxes in glutamate production phase of C. glutamicum, where glutamate flux was 68 with respect to glucose uptake of 100 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.55 |
| glutamate | N/A |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.14 |
| glutamate | 0.03 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 0.17 |
| glutamate | 0.12 |