BMC Syst Biol. 2010 Sep;4:122. A systematic investigation of Escherichia coli central carbon metabolism in response to superoxide stress. Rui B, Shen T, Zhou H, et al.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 91 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 35 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 115 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 97 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli K-12 JM101 |
| Genotype | F- traD36 lacIq delta (lacZ) M15 proA+ B+ supE thi delta (lac-proAB) |
| Culture medium | Batch cultures were grown in 20 ml tubes with 5 ml of LB medium on a gyratory shaker at 200 rpm, at 37 Celsius. continuous cultivations were performed in a modified M9 medium containing 3 g of glucose, 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl,and 30 mM (NH4)2SO4. The following components were sterilized separately and then added (1 ml per liter of final medium): 1 M MgSO4, 0.1 mM CaCl2, 1 mg/mlvitamin B1(filter sterilized). Additionally, 1 ml of polypropylene glycol 2000 was added per liter as an antifoaming agent. Continuous cultivation was performed at 37 Celsius in aerobic chemostats with a working volume of 1.3 liter in a 1.5-liter fermentor (B.Braun Biotech), equipped with pH, dissolved oxygen, and temperature probes. The pH value was maintained at 7.00 by addition of 1 M NaOH, |
| Carbon source | D-Glucose |
| Growth rate | 0.17/h |
| Case-specific description | The central carbon metabolism network and the flux distribution of E.coli grown in normal media. |
| Growth Condition | |
| Strains | Escherichia coli K-12 JM101 |
| Genotype | F- traD36 lacIq delta (lacZ) M15 proA+ B+ supE thi delta (lac-proAB) |
| Culture medium | For continuous cultivation of the wild type E. coli JM101 under superoxide stress conditions, 2 mM paraquat was added using a substrate pump with a constant dilution rate to maintain the final paraquat concentration at 70 uM. |
| Carbon source | D-Glucose |
| Growth rate | 0.17/h |
| Case-specific description | The central carbon metabolism network and the flux distribution of E.coli grown in paraquat-containing minimum media. |
| Specific Rate | (mmol g-1 h-1) |
| 2-Oxoglutarate | 0.84 |
| Pyruvate | 0.04 |
| L-Lactic acid | 0.32 |
| acetate | N/A |
| Specific Rate | (mmol g-1 h-1) |
| 2-Oxoglutarate | 1.73 |
| Pyruvate | 0.02 |
| L-Lactic acid | 0.21 |
| acetate | 4.31 |