J Bacteriol. 2009 Sep;191(17):5538-48. Metabolic flux analysis of Escherichia coli creB and arcA mutants reveals shared control of carbon catabolism under microaerobic growth conditions. Nikel PI, Zhu J, San KY, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 212.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 9.4 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 99.05 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 85.25 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 1.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 91.8 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 1 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli K1060 |
| Genotype | Wild type |
| Culture medium | The minimal salt medium containing (per liter of deionized H2O): 7.0g of Na2HPO4, 3.0g of KH2PO4, 0.5g of NaCl, 1.0g of NH4Cl, 0.2g of MgSO4, 0.01g of CaCl2, and 6mg of thiamine-HCl. MgSO4, CaCl2, and thiamine-HCl were added to the salt medium after autoclaving and cooling as filter-sterilized stock solutions. Foam was suppressed by adding 20 ul of Antifoam B (Sigma-Aldrich, St. Louis, MO) /liter.at the onset of the cultivation. The pH value was controlled at 7.00 by the automatic addition of 3 M NaOH or 1.5 M H2SO4 as required and was double-checked by periodic offline measurements. Unlabeled feeding medium was replaced by an identical salt medium but containing 16 mM unlabeled glucose, 2 mM [U-13C]glucose, and 2 mM [1-13C]glucose |
| Carbon source | D-Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux analysis of E.coli K1060 in glucose-limited continuous cultures with restricted oxygen supply at D=0.1/h. |
| Growth Condition | |
| Strains | Escherichia coli IV1060 |
| Genotype | Same as K1060, but delta creB |
| Culture medium | The minimal salt medium containing (per liter of deionized H2O): 7.0g of Na2HPO4, 3.0g of KH2PO4, 0.5g of NaCl, 1.0g of NH4Cl, 0.2g of MgSO4, 0.01g of CaCl2, and 6mg of thiamine-HCl. MgSO4, CaCl2, and thiamine-HCl were added to the salt medium after autoclaving and cooling as filter-sterilized stock solutions. Foam was suppressed by adding 20 ul of Antifoam B (Sigma-Aldrich, St. Louis, MO) /liter.at the onset of the cultivation. The pH value was controlled at 7.00 by the automatic addition of 3 M NaOH or 1.5 M H2SO4 as required and was double-checked by periodic offline measurements. Unlabeled feeding medium was replaced by an identical salt medium but containing 16 mM unlabeled glucose, 2 mM [U-13C]glucose, and 2 mM [1-13C]glucose |
| Carbon source | D-Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux analysis of IV1060 in glucose-limited continuous cultures with restricted oxygen supply at D=0.1/h. |
| Growth Condition | |
| Strains | Escherichia coli CT1062 |
| Genotype | Same as K1060, but delta arcA |
| Culture medium | The minimal salt medium containing (per liter of deionized H2O): 7.0g of Na2HPO4, 3.0g of KH2PO4, 0.5g of NaCl, 1.0g of NH4Cl, 0.2g of MgSO4, 0.01g of CaCl2, and 6mg of thiamine-HCl. MgSO4, CaCl2, and thiamine-HCl were added to the salt medium after autoclaving and cooling as filter-sterilized stock solutions. Foam was suppressed by adding 20 ul of Antifoam B (Sigma-Aldrich, St. Louis, MO) /liter.at the onset of the cultivation. The pH value was controlled at 7.00 by the automatic addition of 3 M NaOH or 1.5 M H2SO4 as required and was double-checked by periodic offline measurements. Unlabeled feeding medium was replaced by an identical salt medium but containing 16 mM unlabeled glucose, 2 mM [U-13C]glucose, and 2 mM [1-13C]glucose |
| Carbon source | D-Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux analysis of CT1062 in glucose-limited continuous cultures with restricted oxygen supply at D=0.1/h. |
| Growth Condition | |
| Strains | Escherichia coli IV1062 |
| Genotype | Same as CT1062, but delta creB |
| Culture medium | The minimal salt medium containing (per liter of deionized H2O): 7.0g of Na2HPO4, 3.0g of KH2PO4, 0.5g of NaCl, 1.0g of NH4Cl, 0.2g of MgSO4, 0.01g of CaCl2, and 6mg of thiamine-HCl. MgSO4, CaCl2, and thiamine-HCl were added to the salt medium after autoclaving and cooling as filter-sterilized stock solutions. Foam was suppressed by adding 20 ul of Antifoam B (Sigma-Aldrich, St. Louis, MO) /liter.at the onset of the cultivation. The pH value was controlled at 7.00 by the automatic addition of 3 M NaOH or 1.5 M H2SO4 as required and was double-checked by periodic offline measurements. Unlabeled feeding medium was replaced by an identical salt medium but containing 16 mM unlabeled glucose, 2 mM [U-13C]glucose, and 2 mM [1-13C]glucose |
| Carbon source | D-Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux analysis of IV1062 in glucose-limited continuous cultures with restricted oxygen supply at D=0.1/h. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 6.88 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 3.66 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 5.43 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 3.84 |