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J Bacteriol. 2008 Apr;190(7):2323-30. Cyclic AMP-dependent catabolite repression is the dominant control mechanism of metabolic fluxes under glucose limitation in Escherichia coli. Nanchen A, Schicker A, Revelles O, et al.



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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 112 R02736+R01528+R00267+R00214
NADPH production rate Minium 58 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 116 R02736+R01528+R00267+R00214
NADPH production rate Minium 54 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 131 R02736+R01528+R00267+R00214
NADPH production rate Minium 62 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 150 R02736+R01528+R00267+R00214
NADPH production rate Minium 62 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 136 R02736+R01528+R00267+R00214
NADPH production rate Minium 62 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 73 R02736+R01528+R00267+R00214
NADPH production rate Minium 51 R02736+R01528
Growth Condition
Strains Escherichia coli BW25113
Genotype lacIq rrnB3 delta lacZ4787 hsdR514 delta(araBAD)567 delta(rhaBAD)568 rph-1
Culture medium M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient.
Carbon source Glucose
Growth rate 0.1/h
Case-specific description Metabolic net fluxes in the E.coli reference strain in glucose-limited continuous cultures at a dilution rate of 0.1/h.
Growth Condition
Strains Escherichia coli JW0078
Genotype delta cra::kan
Culture medium M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient.
Carbon source Glucose
Growth rate 0.1/h
Case-specific description Metabolic net fluxes in the Cra mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h.
Growth Condition
Strains Escherichia coli JW3320
Genotype crp::kan
Culture medium M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient.
Carbon source Glucose
Growth rate 0.1/h
Case-specific description Metabolic net fluxes in the Crp mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h.
Growth Condition
Strains Escherichia coli JW3778
Genotype delta cya::kan
Culture medium M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient.
Carbon source Glucose
Growth rate 0.1/h
Case-specific description Metabolic net fluxes in the Cya mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h.
Growth Condition
Strains Escherichia coli JW1225
Genotype delta hns::kan
Culture medium M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient.
Carbon source Glucose
Growth rate 0.1/h
Case-specific description Metabolic net fluxes in the the Hns mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h.
Growth Condition
Strains Escherichia coli JW1586
Genotype delta mlc::kan
Culture medium M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient.
Carbon source Glucose
Growth rate 0.1/h
Case-specific description Metabolic net fluxes in the the Mlc mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h.
Specific Rate (mmol g-1 h-1)
glucose 1.1
Specific Rate (mmol g-1 h-1)
glucose 1.2
Specific Rate (mmol g-1 h-1)
glucose 0.8
Specific Rate (mmol g-1 h-1)
glucose 1
Specific Rate (mmol g-1 h-1)
glucose 1.6
Specific Rate (mmol g-1 h-1)
glucose 1.1
Source