J Bacteriol. 2008 Apr;190(7):2323-30. Cyclic AMP-dependent catabolite repression is the dominant control mechanism of metabolic fluxes under glucose limitation in Escherichia coli. Nanchen A, Schicker A, Revelles O, et al.
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ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 112 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 58 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 116 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 54 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 131 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 62 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 150 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 62 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 136 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 62 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 73 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 51 | R02736+R01528 |
Growth Condition | |
Strains | Escherichia coli BW25113 |
Genotype | lacIq rrnB3 delta lacZ4787 hsdR514 delta(araBAD)567 delta(rhaBAD)568 rph-1 |
Culture medium | M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient. |
Carbon source | Glucose |
Growth rate | 0.1/h |
Case-specific description | Metabolic net fluxes in the E.coli reference strain in glucose-limited continuous cultures at a dilution rate of 0.1/h. |
Growth Condition | |
Strains | Escherichia coli JW0078 |
Genotype | delta cra::kan |
Culture medium | M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient. |
Carbon source | Glucose |
Growth rate | 0.1/h |
Case-specific description | Metabolic net fluxes in the Cra mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h. |
Growth Condition | |
Strains | Escherichia coli JW3320 |
Genotype | crp::kan |
Culture medium | M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient. |
Carbon source | Glucose |
Growth rate | 0.1/h |
Case-specific description | Metabolic net fluxes in the Crp mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h. |
Growth Condition | |
Strains | Escherichia coli JW3778 |
Genotype | delta cya::kan |
Culture medium | M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient. |
Carbon source | Glucose |
Growth rate | 0.1/h |
Case-specific description | Metabolic net fluxes in the Cya mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h. |
Growth Condition | |
Strains | Escherichia coli JW1225 |
Genotype | delta hns::kan |
Culture medium | M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient. |
Carbon source | Glucose |
Growth rate | 0.1/h |
Case-specific description | Metabolic net fluxes in the the Hns mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h. |
Growth Condition | |
Strains | Escherichia coli JW1586 |
Genotype | delta mlc::kan |
Culture medium | M9 minimal medium that contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4-2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter final medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3-6H2O, 0.18 g of ZnSO4-7H2O, 0.12 g of CuCl2-2H2O, 0.12 g of MnSO4-H2O, and 0.18 g of CoCl2-6H2O.Sterilized glucose was added to a final concentration of 1 g/liter as the limiting nutrient. |
Carbon source | Glucose |
Growth rate | 0.1/h |
Case-specific description | Metabolic net fluxes in the the Mlc mutants in glucose-limited continuous cultures at a dilution rate of 0.1/h. |
Specific Rate | (mmol g-1 h-1) |
glucose | 1.1 |
Specific Rate | (mmol g-1 h-1) |
glucose | 1.2 |
Specific Rate | (mmol g-1 h-1) |
glucose | 0.8 |
Specific Rate | (mmol g-1 h-1) |
glucose | 1 |
Specific Rate | (mmol g-1 h-1) |
glucose | 1.6 |
Specific Rate | (mmol g-1 h-1) |
glucose | 1.1 |