J Bacteriol. 2003 Dec;185(24):7053-67. Responses of the central metabolism in Escherichia coli to phosphoglucose isomerase and glucose-6-phosphate dehydrogenase knockouts. Hua Q, Yang C, Baba T, et al.
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| ATP production rate Maximum | 1053 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 813 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 154 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 58 | R02736+R01528 |
| ATP production rate Maximum | 1044.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 814.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 114 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 22 | R02736+R01528 |
| ATP production rate Maximum | 704.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 639.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 219 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 193 | R02736+R01528 |
| ATP production rate Maximum | 702 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 592 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 217 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 173 | R02736+R01528 |
| ATP production rate Maximum | 1099 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 849 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 100 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| ATP production rate Maximum | 463 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 353 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 44 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli W3110 |
| Genotype | F- Lambda- IN(rrnD-rrnE)1 rph-1 |
| Culture medium | The minimal medium contained (perliter): 5.0g of glucose, 1.0g of NH4Cl, 2.7g of (NH4)2SO4, 6.8g of Na2HPO4, 3.0g of KH2PO4, 0.6g of NaCl, 0.2g of MgSO4-7H2O, 1.0ug of thiamine HCl, 2.0ul of polypropylene glycol 2000 as an antifoaming agent, and 10 ml of a trace element solution containing (per liter) 0.55g of CaCl2-2H2O, 1.67g of FeCl3-6H2O, 0.10g of MnCl4-4H2O, 0.17g of ZnCl2, 0.04g of CuCl2-2H2O, 0.06g of CoCl2-6H2O, and 0.06g of Na2MoO4-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux distribution in chemostat cultures of E.coli W3110 under glucose-limited conditions. |
| Growth Condition | |
| Strains | Escherichia coli W3110 |
| Genotype | F- Lambda- IN(rrnD-rrnE)1 rph-1 |
| Culture medium | The minimal medium contained (perliter): 5.0g of glucose, 1.0g of NH4Cl, 2.7g of (NH4)2SO4, 6.8g of Na2HPO4, 3.0g of KH2PO4, 0.6g of NaCl, 0.2g of MgSO4-7H2O, 1.0ug of thiamine HCl, 2.0ul of polypropylene glycol 2000 as an antifoaming agent, and 10 ml of a trace element solution containing (per liter) 0.55g of CaCl2-2H2O, 1.67g of FeCl3-6H2O, 0.10g of MnCl4-4H2O, 0.17g of ZnCl2, 0.04g of CuCl2-2H2O, 0.06g of CoCl2-6H2O, and 0.06g of Na2MoO4-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux distribution in chemostat cultures of E.coli W3110 under ammonia-limited conditions. |
| Growth Condition | |
| Strains | Escherichia coli JWK3985 |
| Genotype | pgi gene knock-out |
| Culture medium | The minimal medium contained (perliter): 5.0g of glucose, 1.0g of NH4Cl, 2.7g of (NH4)2SO4, 6.8g of Na2HPO4, 3.0g of KH2PO4, 0.6g of NaCl, 0.2g of MgSO4-7H2O, 1.0ug of thiamine HCl, 2.0ul of polypropylene glycol 2000 as an antifoaming agent, and 10 ml of a trace element solution containing (per liter) 0.55g of CaCl2-2H2O, 1.67g of FeCl3-6H2O, 0.10g of MnCl4-4H2O, 0.17g of ZnCl2, 0.04g of CuCl2-2H2O, 0.06g of CoCl2-6H2O, and 0.06g of Na2MoO4-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux distribution in chemostat cultures of the Pgi mutant under glucose-limited conditions. |
| Growth Condition | |
| Strains | Escherichia coli JWK3985 |
| Genotype | pgi gene knock-out |
| Culture medium | The minimal medium contained (perliter): 5.0g of glucose, 1.0g of NH4Cl, 2.7g of (NH4)2SO4, 6.8g of Na2HPO4, 3.0g of KH2PO4, 0.6g of NaCl, 0.2g of MgSO4-7H2O, 1.0ug of thiamine HCl, 2.0ul of polypropylene glycol 2000 as an antifoaming agent, and 10 ml of a trace element solution containing (per liter) 0.55g of CaCl2-2H2O, 1.67g of FeCl3-6H2O, 0.10g of MnCl4-4H2O, 0.17g of ZnCl2, 0.04g of CuCl2-2H2O, 0.06g of CoCl2-6H2O, and 0.06g of Na2MoO4-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux distribution in chemostat cultures of the Pgi mutant under ammonia-limited conditions. |
| Growth Condition | |
| Strains | Escherichia coli JWK1841 |
| Genotype | zwf gene knock-out |
| Culture medium | The minimal medium contained (perliter): 5.0g of glucose, 1.0g of NH4Cl, 2.7g of (NH4)2SO4, 6.8g of Na2HPO4, 3.0g of KH2PO4, 0.6g of NaCl, 0.2g of MgSO4-7H2O, 1.0ug of thiamine HCl, 2.0ul of polypropylene glycol 2000 as an antifoaming agent, and 10 ml of a trace element solution containing (per liter) 0.55g of CaCl2-2H2O, 1.67g of FeCl3-6H2O, 0.10g of MnCl4-4H2O, 0.17g of ZnCl2, 0.04g of CuCl2-2H2O, 0.06g of CoCl2-6H2O, and 0.06g of Na2MoO4-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux distribution in chemostat cultures of the Zwf mutant under glucose-limited conditions. |
| Growth Condition | |
| Strains | Escherichia coli JWK1841 |
| Genotype | zwf gene knock-out |
| Culture medium | The minimal medium contained (perliter): 5.0g of glucose, 1.0g of NH4Cl, 2.7g of (NH4)2SO4, 6.8g of Na2HPO4, 3.0g of KH2PO4, 0.6g of NaCl, 0.2g of MgSO4-7H2O, 1.0ug of thiamine HCl, 2.0ul of polypropylene glycol 2000 as an antifoaming agent, and 10 ml of a trace element solution containing (per liter) 0.55g of CaCl2-2H2O, 1.67g of FeCl3-6H2O, 0.10g of MnCl4-4H2O, 0.17g of ZnCl2, 0.04g of CuCl2-2H2O, 0.06g of CoCl2-6H2O, and 0.06g of Na2MoO4-4H2O. |
| Carbon source | Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Metabolic flux distribution in chemostat cultures of the Zwf mutant under ammonia-limited conditions. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.4 |
| O2 | 4 |
| CO2 | 4.2 |
| Acetate | 0 |
| Pyruvate | 0 |
| Ethanol | 0 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.3 |
| O2 | 3.9 |
| CO2 | 3.7 |
| Acetate | 0 |
| Pyruvate | 0 |
| Ethanol | 0 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.4 |
| O2 | 3.7 |
| CO2 | 4.2 |
| Acetate | 0 |
| Pyruvate | 0 |
| Ethanol | 0 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.9 |
| O2 | 7.1 |
| CO2 | 7.2 |
| Acetate | 1.6 |
| Pyruvate | 0.01 |
| Ethanol | 0.02 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.6 |
| O2 | 3.8 |
| CO2 | 4 |
| Acetate | 0.31 |
| Pyruvate | 0 |
| Ethanol | 0 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 2.8 |
| O2 | 3 |
| CO2 | 3.1 |
| Acetate | 2.71 |
| Pyruvate | 0.71 |
| Ethanol | 0.03 |