Microbiology. 2006 Aug;152(Pt 8):2421-31. Indole-3-acetic acid regulates the central metabolic pathways in Escherichia coli. Bianco C, Imperlini E, Calogero R, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli K-12 MG1655 |
| Genotype | |
| Culture medium | Cells were grown aerobically at 37 Celsius in M9 minimal medium containing 20 mg uracil, 1 mg thiamine, 0.4% L-arabinose, glucose, mannitol, lactose or glycerol as carbon source, and supplemented with 40mg casein acid hydrolysate.Solid media contained 15 g agar (Difco) in TY (0.5% yeast extract, 0.8% NaCl and 1% tryptone) or minimalmedium. Exponentially growing E.coli K-12 MG1655 cultures(OD600 0.6) were split into two aliquots; to the first one, an IAA solution was added to a final concentration of 0.5 mM and the other was left untreated (control). |
| Carbon source | D-Glucose |
| Growth rate | |
| Case-specific description | Activities of central metabolic pathway enzymes in IAA-treated cells compared with those of control cells. |
| Specific Rate | (mmol g-1 h-1) |