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Microbiology. 2006 Aug;152(Pt 8):2421-31. Indole-3-acetic acid regulates the central metabolic pathways in Escherichia coli. Bianco C, Imperlini E, Calogero R, et al.

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ATP production rate Maximum 2015.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
ATP production rate Minium 1215.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
Growth Condition
Strains Escherichia coli K-12 MG1655
Culture medium Cells were grown aerobically at 37 Celsius in M9 minimal medium containing 20 mg uracil, 1 mg thiamine, 0.4% L-arabinose, glucose, mannitol, lactose or glycerol as carbon source, and supplemented with 40mg casein acid hydrolysate.Solid media contained 15 g agar (Difco) in TY (0.5% yeast extract, 0.8% NaCl and 1% tryptone) or minimalmedium. Exponentially growing E.coli K-12 MG1655 cultures(OD600 0.6) were split into two aliquots; to the first one, an IAA solution was added to a final concentration of 0.5 mM and the other was left untreated (control).
Carbon source D-Glucose
Growth rate
Case-specific description Activities of central metabolic pathway enzymes in IAA-treated cells compared with those of control cells.
Specific Rate (mmol g-1 h-1)