Microb Cell Fact. 2012 Sep;11:127. Consequences of phosphoenolpyruvate:sugar phosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli. Meza E, Becker J, Bolivar F, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 522.45 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 30.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 900.3 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 52.7 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 416.2 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 52 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 680.95 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 48.6 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli W3110 |
| Genotype | F- lambda- INV(rrnD - rrnE)1 |
| Culture medium | The medium used during mutant strains construction and selection was Luria-Bertani (LB) with the corresponding antibiotic: Cm 10 ug/ml, Km 10 ug/ml and Gm 5 ug/ml. In shake flask cultures and labeling experiment,minimal medium was used. It contained glucose as the sole carbon source at a concentration of 10 g/l, K2HPO4 90 mM, KH2PO4 10 mM, (NH4)2SO4 40 mM, NaCl 20 mM, MgSO4-2H2O 1.6 mM, CaCl2 0.05 mM, FeSO4-7H2O 0.072 mM and vitamin B1 0.05 mM. Glucose and salts solution were sterilized separately at 121 Celsius during 20 minutes and vitamin B1 was sterilized by filtration. All stock cultures were stored at 70 Celsius below in LB medium containing glycerol (25% v/v). |
| Carbon source | D-Glucose |
| Growth rate | 0.69/h |
| Case-specific description | Metabolic flux distribution and Pyk activities of strains W3110(reference). |
| Growth Condition | |
| Strains | Escherichia coli VH33 |
| Genotype | W3110[delta ptsI,ptsH,crr::kan delta lacI;lacZ::loxP] galP+ delta PgalP::PTrc |
| Culture medium | The medium used during mutant strains construction and selection was Luria-Bertani (LB) with the corresponding antibiotic: Cm 10 ug/ml, Km 10 ug/ml and Gm 5 ug/ml. In shake flask cultures and labeling experiment,minimal medium was used. It contained glucose as the sole carbon source at a concentration of 10 g/l, K2HPO4 90 mM, KH2PO4 10 mM, (NH4)2SO4 40 mM, NaCl 20 mM, MgSO4-2H2O 1.6 mM, CaCl2 0.05 mM, FeSO4-7H2O 0.072 mM and vitamin B1 0.05 mM. Glucose and salts solution were sterilized separately at 121 Celsius during 20 minutes and vitamin B1 was sterilized by filtration. All stock cultures were stored at 70 Celsius below in LB medium containing glycerol (25% v/v). |
| Carbon source | D-Glucose |
| Growth rate | 0.45/h |
| Case-specific description | Metabolic flux distribution and Pyk activities of Escherichia coli VH33(PTS- glc+). |
| Growth Condition | |
| Strains | Escherichia coli VH34 |
| Genotype | VH33 pykA::cat |
| Culture medium | The medium used during mutant strains construction and selection was Luria-Bertani (LB) with the corresponding antibiotic: Cm 10 ug/ml, Km 10 ug/ml and Gm 5 ug/ml. In shake flask cultures and labeling experiment,minimal medium was used. It contained glucose as the sole carbon source at a concentration of 10 g/l, K2HPO4 90 mM, KH2PO4 10 mM, (NH4)2SO4 40 mM, NaCl 20 mM, MgSO4-2H2O 1.6 mM, CaCl2 0.05 mM, FeSO4-7H2O 0.072 mM and vitamin B1 0.05 mM. Glucose and salts solution were sterilized separately at 121 Celsius during 20 minutes and vitamin B1 was sterilized by filtration. All stock cultures were stored at 70 Celsius below in LB medium containing glycerol (25% v/v). |
| Carbon source | D-Glucose |
| Growth rate | 0.44/h |
| Case-specific description | Metabolic flux distribution and Pyk activities of Escherichia coli VH34(PTS- glc+ pykA-). |
| Growth Condition | |
| Strains | Escherichia coli VH35 |
| Genotype | VH33 pykF::gen |
| Culture medium | The medium used during mutant strains construction and selection was Luria-Bertani (LB) with the corresponding antibiotic: Cm 10 ug/ml, Km 10 ug/ml and Gm 5 ug/ml. In shake flask cultures and labeling experiment,minimal medium was used. It contained glucose as the sole carbon source at a concentration of 10 g/l, K2HPO4 90 mM, KH2PO4 10 mM, (NH4)2SO4 40 mM, NaCl 20 mM, MgSO4-2H2O 1.6 mM, CaCl2 0.05 mM, FeSO4-7H2O 0.072 mM and vitamin B1 0.05 mM. Glucose and salts solution were sterilized separately at 121 Celsius during 20 minutes and vitamin B1 was sterilized by filtration. All stock cultures were stored at 70 Celsius below in LB medium containing glycerol (25% v/v). |
| Carbon source | D-Glucose |
| Growth rate | 0.36/h |
| Case-specific description | Metabolic flux distribution and Pyk activities of Escherichia coli VH35(PTS- glc+ pykF-). |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 16.1 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 6.9 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.2 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.2 |