J Biotechnol. 2007 Jan;128(1):93-111. Determination of metabolic flux changes during fed-batch cultivation from measurements of intracellular amino acids by LC-MS/MS. Iwatani S, Van Dien S, Shimbo K, et al.
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| ATP production rate Maximum | 1301.55 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 1062.3 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 140.5 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 44.8 | R02736+R01528 |
| ATP production rate Maximum | 1157.75 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 1043 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 105.1 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 59.2 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli WYK050 |
| Genotype | pCAB |
| Culture medium | Th lysine-producing strain was cultivate overnight at 37 Celsius on LB plates containing 1.0% Bacto tryptone, 0.5% Bacto yeast extract, 1% NaCl and 1.5% agar. The cells collected from two incubated plates were inoculated into culture medium, which contained 16g/L of (NH4)2SO4, 3g/L of KH2PO4, 4g/L of yeast extract, 10mg/L of FeSO4-7H2O, 10mg/L of MnSO4-5H2O, 400mg/L of isoleucine, 40g/L of 13C-labeled glucose and 1g/L of MgSO4-7H2O.Tracer experiments for metabolic flux analysis of E.coli WYK050/pCAB1 were carried out in a 1-L jar fermentor with a working volume of 300mL using a mixture of 9.6g of [1-13C] glucose and 2.4g of [U-13C] glucose as a carbon source. The temperature was controlled at 37 Celsius, and the pH was set at 6.7 using NH3 gas. |
| Carbon source | D-Glucose |
| Growth rate | 0.11/h |
| Case-specific description | Metabolic fluxes in the central metabolism and amino acid biosynthetic pathways of E.coli WYK050/pCAB1 during exponential growth (CT 17h). |
| Growth Condition | |
| Strains | Escherichia coli WYK050 |
| Genotype | pCAB |
| Culture medium | Th lysine-producing strain was cultivate overnight at 37 Celsius on LB plates containing 1.0% Bacto tryptone, 0.5% Bacto yeast extract, 1% NaCl and 1.5% agar. The cells collected from two incubated plates were inoculated into culture medium, which contained 16g/L of (NH4)2SO4, 3g/L of KH2PO4, 4g/L of yeast extract, 10mg/L of FeSO4-7H2O, 10mg/L of MnSO4-5H2O, 400mg/L of isoleucine, 40g/L of 13C-labeled glucose and 1g/L of MgSO4-7H2O.Tracer experiments for metabolic flux analysis of E.coli WYK050/pCAB1 were carried out in a 1-L jar fermentor with a working volume of 300mL using a mixture of 9.6g of [1-13C] glucose and 2.4g of [U-13C] glucose as a carbon source. The temperature was controlled at 37 Celsius, and the pH was set at 6.7 using NH3 gas. |
| Carbon source | D-Glucose |
| Growth rate | 0.01/h |
| Case-specific description | Metabolic fluxes in the central metabolism and amino acid biosynthetic pathways of lysine-producing E.coli WYK050/pCAB1 in the stationary phase (CT 26h). |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |