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J Bacteriol. 2005 May;187(9):3171-9. Impact of global transcriptional regulation by ArcA, ArcB, Cra, Crp, Cya, Fnr, and Mlc on glucose catabolism in Escherichia coli. Perrenoud A, Sauer U.



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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
Growth Condition
Strains Escherichia coli BW25113
Genotype lacIqrrnB3 deltalacZ4787 hsdR514 delta(araBAD)567 delta(rhaBAD)568 rph-1
Culture medium M9 medium contained (per liter of deionized water) 0.8g of NH4Cl, 0.5g of NaCl, 7.5g of Na2HPO4-2H2O, and 3.0g of KH2PO4. The following components were sterilized separately and then added (per liter [final volume] of medium): 2ml of 1M MgSO4, 1ml of 0.1M CaCl2, 0.3ml of 1 mM filter sterilized thiamine HCl, and 10ml of a trace element solution containing (perliter) 1g of FeCl3-6H2O, 0.18g of ZnSO4-7H2O, 0.12g of CuCl2-2H2O, 0.12g of MnSO4-H2O, and 0.18g of CoCl2-6H2O. Sterilized glucose was added to a final concentration of 2 or 3 g per liter. For 13C-labeling experiments, glucose was added either entirely as the 1-13C-labeled isotope isomer (>99%; Euriso-top,GIF-sur-Yvette, France) or as a mixture of 20% (wt/wt) [U-13C]glucose (13C,>98%; Isotech, Miamisburg, Ohio) and 80% (wt/wt) natural glucose.
Carbon source D-Glucose
Growth rate 0.65/h
Case-specific description Metabolic flux distribution in aerobic batch culture of the parent strain,with 100% [1-13C]glucose and with a mixture of 20% [U-13C]glucose and 80% unlabeled glucose.
Growth Condition
Strains Escherichia coli BW25113
Genotype lacIqrrnB3 deltalacZ4787 hsdR514 delta(araBAD)567 delta(rhaBAD)568 rph-1
Culture medium M9 medium contained (per liter of deionized water) 0.8g of NH4Cl, 0.5g of NaCl, 7.5g of Na2HPO4-2H2O, and 3.0g of KH2PO4. The following components were sterilized separately and then added (per liter [final volume] of medium): 2ml of 1M MgSO4, 1ml of 0.1M CaCl2, 0.3ml of 1 mM filter sterilized thiamine HCl, and 10ml of a trace element solution containing (perliter) 1g of FeCl3-6H2O, 0.18g of ZnSO4-7H2O, 0.12g of CuCl2-2H2O, 0.12g of MnSO4-H2O, and 0.18g of CoCl2-6H2O. Sterilized glucose was added to a final concentration of 2 or 3 g per liter. For 13C-labeling experiments, glucose was added either entirely as the 1-13C-labeled isotope isomer (>99%; Euriso-top,GIF-sur-Yvette, France) or as a mixture of 20% (wt/wt) [U-13C]glucose (13C,>98%; Isotech, Miamisburg, Ohio) and 80% (wt/wt) natural glucose.
Carbon source D-Glucose
Growth rate 0.60/h
Case-specific description Metabolic flux distribution in anaerobic batch culture of the parent strain,with 100% [1-13C]glucose and with a mixture of 20% [U-13C]glucose and 80% unlabeled glucose.
Growth Condition
Strains Escherichia coli BW25113
Genotype lacIqrrnB3 deltalacZ4787 hsdR514 delta(araBAD)567 delta(rhaBAD)568 rph-1
Culture medium M9 medium contained (per liter of deionized water) 0.8g of NH4Cl, 0.5g of NaCl, 7.5g of Na2HPO4-2H2O, and 3.0g of KH2PO4. The following components were sterilized separately and then added (per liter [final volume] of medium): 2ml of 1M MgSO4, 1ml of 0.1M CaCl2, 0.3ml of 1 mM filter sterilized thiamine HCl, and 10ml of a trace element solution containing (perliter) 1g of FeCl3-6H2O, 0.18g of ZnSO4-7H2O, 0.12g of CuCl2-2H2O, 0.12g of MnSO4-H2O, and 0.18g of CoCl2-6H2O. Sterilized glucose was added to a final concentration of 2 or 3 g per liter. For 13C-labeling experiments, glucose was added either entirely as the 1-13C-labeled isotope isomer (>99%; Euriso-top,GIF-sur-Yvette, France) or as a mixture of 20% (wt/wt) [U-13C]glucose (13C,>98%; Isotech, Miamisburg, Ohio) and 80% (wt/wt) natural glucose.
Carbon source D-Glucose
Growth rate 0.61/h
Case-specific description Metabolic flux distribution in anaerobic nitrate-respiring batch culture of the parent strain,with 100% [1-13C]glucose and with a mixture of 20% [U-13C]glucose and 80% unlabeled glucose.
Growth Condition
Strains Escherichia coli JW4364 (ArcA mutant)
Genotype BW25113 delta arcA::kan
Culture medium M9 medium contained (per liter of deionized water) 0.8g of NH4Cl, 0.5g of NaCl, 7.5g of Na2HPO4-2H2O, and 3.0g of KH2PO4. The following components were sterilized separately and then added (per liter [final volume] of medium): 2ml of 1M MgSO4, 1ml of 0.1M CaCl2, 0.3ml of 1 mM filter sterilized thiamine HCl, and 10ml of a trace element solution containing (perliter) 1g of FeCl3-6H2O, 0.18g of ZnSO4-7H2O, 0.12g of CuCl2-2H2O, 0.12g of MnSO4-H2O, and 0.18g of CoCl2-6H2O. Sterilized glucose was added to a final concentration of 2 or 3 g per liter. For 13C-labeling experiments, glucose was added either entirely as the 1-13C-labeled isotope isomer (>99%; Euriso-top,GIF-sur-Yvette, France) or as a mixture of 20% (wt/wt) [U-13C]glucose (13C,>98%; Isotech, Miamisburg, Ohio) and 80% (wt/wt) natural glucose.
Carbon source D-Glucose
Growth rate 0.65/h
Case-specific description Metabolic flux distribution in aerobic batch culture of the ArcA mutant,with 100% [1-13C]glucose and with a mixture of 20% [U-13C]glucose and 80% unlabeled glucose.
Growth Condition
Strains Escherichia coli JW4364 (ArcA mutant)
Genotype BW25113 delta arcA::kan
Culture medium M9 medium contained (per liter of deionized water) 0.8g of NH4Cl, 0.5g of NaCl, 7.5g of Na2HPO4-2H2O, and 3.0g of KH2PO4. The following components were sterilized separately and then added (per liter [final volume] of medium): 2ml of 1M MgSO4, 1ml of 0.1M CaCl2, 0.3ml of 1 mM filter sterilized thiamine HCl, and 10ml of a trace element solution containing (perliter) 1g of FeCl3-6H2O, 0.18g of ZnSO4-7H2O, 0.12g of CuCl2-2H2O, 0.12g of MnSO4-H2O, and 0.18g of CoCl2-6H2O. Sterilized glucose was added to a final concentration of 2 or 3 g per liter. For 13C-labeling experiments, glucose was added either entirely as the 1-13C-labeled isotope isomer (>99%; Euriso-top,GIF-sur-Yvette, France) or as a mixture of 20% (wt/wt) [U-13C]glucose (13C,>98%; Isotech, Miamisburg, Ohio) and 80% (wt/wt) natural glucose.
Carbon source D-Glucose
Growth rate 0.25/h
Case-specific description Metabolic flux distribution in anaerobic batch culture of the ArcA mutant,with 100% [1-13C]glucose and with a mixture of 20% [U-13C]glucose and 80% unlabeled glucose.
Growth Condition
Strains Escherichia coli JW4364 (ArcA mutant)
Genotype BW25113 delta arcA::kan
Culture medium M9 medium contained (per liter of deionized water) 0.8g of NH4Cl, 0.5g of NaCl, 7.5g of Na2HPO4-2H2O, and 3.0g of KH2PO4. The following components were sterilized separately and then added (per liter [final volume] of medium): 2ml of 1M MgSO4, 1ml of 0.1M CaCl2, 0.3ml of 1 mM filter sterilized thiamine HCl, and 10ml of a trace element solution containing (perliter) 1g of FeCl3-6H2O, 0.18g of ZnSO4-7H2O, 0.12g of CuCl2-2H2O, 0.12g of MnSO4-H2O, and 0.18g of CoCl2-6H2O. Sterilized glucose was added to a final concentration of 2 or 3 g per liter. For 13C-labeling experiments, glucose was added either entirely as the 1-13C-labeled isotope isomer (>99%; Euriso-top,GIF-sur-Yvette, France) or as a mixture of 20% (wt/wt) [U-13C]glucose (13C,>98%; Isotech, Miamisburg, Ohio) and 80% (wt/wt) natural glucose.
Carbon source D-Glucose
Growth rate 0.27/h
Case-specific description Metabolic flux distribution in anaerobic nitrate-respiring batch culture of the ArcA mutant,with 100% [1-13C]glucose and with a mixture of 20% [U-13C]glucose and 80% unlabeled glucose.
Growth Condition
Strains JW3320 (Crp mutant)
Genotype BW25113 delta crp::kan
Culture medium M9 medium contained (per liter of deionized water) 0.8g of NH4Cl, 0.5g of NaCl, 7.5g of Na2HPO4-2H2O, and 3.0g of KH2PO4. The following components were sterilized separately and then added (per liter [final volume] of medium): 2ml of 1M MgSO4, 1ml of 0.1M CaCl2, 0.3ml of 1 mM filter sterilized thiamine HCl, and 10ml of a trace element solution containing (perliter) 1g of FeCl3-6H2O, 0.18g of ZnSO4-7H2O, 0.12g of CuCl2-2H2O, 0.12g of MnSO4-H2O, and 0.18g of CoCl2-6H2O. Sterilized glucose was added to a final concentration of 2 or 3 g per liter. For 13C-labeling experiments, glucose was added either entirely as the 1-13C-labeled isotope isomer (>99%; Euriso-top,GIF-sur-Yvette, France) or as a mixture of 20% (wt/wt) [U-13C]glucose (13C,>98%; Isotech, Miamisburg, Ohio) and 80% (wt/wt) natural glucose.
Carbon source D-Glucose
Growth rate 0.61/h
Case-specific description Metabolic flux distribution in the Crp mutant during exponential aerobic growth , with 100% [1-13C]glucose and with a mixture of 20% [U-13C]glucose and 80% unlabeled glucose.
Growth Condition
Strains JW3778 (Cya mutant)
Genotype BW25113 delta cya::kan
Culture medium M9 medium contained (per liter of deionized water) 0.8g of NH4Cl, 0.5g of NaCl, 7.5g of Na2HPO4-2H2O, and 3.0g of KH2PO4. The following components were sterilized separately and then added (per liter [final volume] of medium): 2ml of 1M MgSO4, 1ml of 0.1M CaCl2, 0.3ml of 1 mM filter sterilized thiamine HCl, and 10ml of a trace element solution containing (perliter) 1g of FeCl3-6H2O, 0.18g of ZnSO4-7H2O, 0.12g of CuCl2-2H2O, 0.12g of MnSO4-H2O, and 0.18g of CoCl2-6H2O. Sterilized glucose was added to a final concentration of 2 or 3 g per liter. For 13C-labeling experiments, glucose was added either entirely as the 1-13C-labeled isotope isomer (>99%; Euriso-top,GIF-sur-Yvette, France) or as a mixture of 20% (wt/wt) [U-13C]glucose (13C,>98%; Isotech, Miamisburg, Ohio) and 80% (wt/wt) natural glucose.
Carbon source D-Glucose
Growth rate 0.60/h
Case-specific description Metabolic flux distribution in the Cya mutant during exponential aerobic growth,with 100% [1-13C]glucose and with a mixture of 20% [U-13C]glucose and 80% unlabeled glucose.
Specific Rate (mmol g-1 h-1)
glucose 7.6
acetate 4.8
Specific Rate (mmol g-1 h-1)
glucose 7.2
acetate 3.5
Specific Rate (mmol g-1 h-1)
glucose 7.4
acetate 4.9
Specific Rate (mmol g-1 h-1)
glucose 8.1
acetate 5.7
Specific Rate (mmol g-1 h-1)
glucose 2.5
acetate 0.7
Specific Rate (mmol g-1 h-1)
glucose 2.7
acetate 0.8
Specific Rate (mmol g-1 h-1)
glucose 7.7
acetate 4.8
Specific Rate (mmol g-1 h-1)
glucose 7.1
acetate 4.3
Source